Bredeweg Erin, Orr Galya, Hu Dehong
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, USA.
Curr Res Microb Sci. 2025 Mar 2;8:100369. doi: 10.1016/j.crmicr.2025.100369. eCollection 2025.
In this report, we present coordinated observations of protein and mRNA transcript counts at the single-cell level in the oleaginous yeast model . The transcription factor Xbp1p regulates entry into a quiescent state, representing a shift of resources to sequestration of nutrients rather than cell division. We observed the responses of wild-type and Δ cells to protein (by fluorescence) and transcript quantification and localization at both single-cell and population-averaged levels. Data were collected via single-molecule fluorescence hybridization (smFISH) and qPCR under nitrogen depletion, a condition that drives lipid accumulation. These techniques reveal a complex and heterogeneous population of Xbp1p dynamics and downstream regulation. Our findings highlight the need for single-cell resolution analyses to describe cellular dynamics and regulatory processes.
在本报告中，我们展示了在产油酵母模型中对单细胞水平的蛋白质和mRNA转录本计数进行的协同观察。转录因子Xbp1p调节进入静止状态，这代表着资源从细胞分裂转向营养物质的储存。我们在单细胞和群体平均水平上观察了野生型和Δ细胞对蛋白质（通过荧光）以及转录本定量和定位的反应。在氮耗尽（一种驱动脂质积累的条件）下，通过单分子荧光杂交（smFISH）和qPCR收集数据。这些技术揭示了Xbp1p动态和下游调控的复杂且异质的群体。我们的研究结果强调了需要单细胞分辨率分析来描述细胞动态和调控过程。
