Zhang Yan, Liang Shuang, Choi Seongho, Li Guangyu
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin 132109, China.
J Anim Sci Technol. 2025 May;67(3):619-635. doi: 10.5187/jast.2024.e37. Epub 2025 May 31.
This study investigated whether the microbial assemblages in the gastrointestinal tracts (GITs) of sika deer calves can be manipulated by maternal rumen microbiota transplantation (MRMT). The results suggest that MRMT had no significant effect on the growth of calves but markedly lowered the duration of diarrhea and increased rumen fermentation in sika deer calves. Sequencing analysis of 16S rRNA gene amplicons revealed that MRMT increased the ability of some microbial taxa to colonize the GIT or enabled the colonization of others, which caused the ruminal microbial communities in sika deer calves to shift such that they resembled those of their mothers and promoted the temporal development of gut microbial diversity in deer calves. Moreover, after inoculation, 7 inoculum-dominant taxa (, , , , , , and ) and one inoculum-dominant taxon () were significantly enriched in the rumen and feces of the sika deer calves, respectively. These data suggest that MRMT may be an effective approach for promoting microbial establishment in the GIT and preventing diarrhea in sika deer calves.
本研究调查了母羊瘤胃微生物群移植（MRMT）是否能调控梅花鹿幼崽胃肠道（GIT）中的微生物群落。结果表明，MRMT对幼崽生长无显著影响，但显著缩短了梅花鹿幼崽的腹泻持续时间并增强了瘤胃发酵。对16S rRNA基因扩增子的测序分析表明，MRMT增强了一些微生物类群在GIT中定殖的能力或使其他微生物类群得以定殖，这导致梅花鹿幼崽瘤胃微生物群落发生变化，使其类似于母鹿的群落，并促进了幼鹿肠道微生物多样性的时序发育。此外，接种后，7个接种物优势类群（、、、、、、和）和1个接种物优势类群（）分别在梅花鹿幼崽的瘤胃和粪便中显著富集。这些数据表明，MRMT可能是促进梅花鹿幼崽GIT中微生物定植和预防腹泻的有效方法。
