Chen Ji, Zhang Fan, Chen Yangyuxi, Lu Yingqing, Liu Xinxin, Tang Yuanzhang, Yang Fengrui
Department of Endocrinology Hunan University of Medicine General Hospital Huaihua Hunan China.
Department of Anesthesiology Xiangya Hospital of Central South University Changsha Hunan Province China.
J Cell Commun Signal. 2025 Jun 12;19(2):e70019. doi: 10.1002/ccs3.70019. eCollection 2025 Jun.
Diabetic peripheral neuropathy (DPN), a common complication of diabetes mellitus, involves complex molecular pathways and the ceRNA regulatory network. Integration of diabetes-related circRNA datasets identified circ_0012856 as pivotal via least absolute shrinkage and selection operator and support vector machine recursive feature elimination algorithms. Functional enrichment analyses elucidated its involvement in DPN pathogenesis. In vitro studies showed circ_0012856 regulating EZH2 and STAT3 expressions, inhibiting autophagy, and promoting microglial M1 polarization. In vivo, experiments revealed silencing circ_0012856 alleviating DPN symptoms in diabetic mice. Overall, circ_0012856 acts as a miR-124 sponge, affecting key pathways in DPN progression and providing potential therapeutic targets.
糖尿病周围神经病变（DPN）是糖尿病的常见并发症，涉及复杂的分子途径和ceRNA调控网络。通过最小绝对收缩和选择算子以及支持向量机递归特征消除算法，整合糖尿病相关的circRNA数据集，确定circ_0012856为关键因素。功能富集分析阐明了其在DPN发病机制中的作用。体外研究表明，circ_0012856调节EZH2和STAT3的表达，抑制自噬，并促进小胶质细胞M1极化。在体内，实验表明沉默circ_0012856可减轻糖尿病小鼠的DPN症状。总体而言，circ_0012856作为miR-124的海绵，影响DPN进展中的关键途径，并提供潜在的治疗靶点。
