生成NOD2配体的肽聚糖d，l-内肽酶BbMep的生化特性

Biochemical characterization of peptidoglycan d,l-endopeptidase BbMep that generates NOD2 ligands.

作者信息

Kwan Jeric Mun Chung, Feng Shiliu, Ng Evan Wei Long, Qiao Yuan

机构信息

School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University 21 Nanyang Link 637371 Singapore

Lee Kong Chian School of Medicine, Nanyang Technological University 11 Mandalay Road 308232 Singapore.

出版信息

RSC Chem Biol. 2025 May 30. doi: 10.1039/d5cb00086f.

DOI:10.1039/d5cb00086f

PMID:40520142

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12160582/

Abstract

Soluble peptidoglycan fragments produced by the gut bacteria are key effectors in microbiota-host crosstalk. Here, we biochemically characterized BbMep, an NlpC/p60 domain-containing peptidoglycan d,l-endopeptidase from , which efficiently digests Lys- or Orn-type sacculi. Digestion of human stool-derived muropeptides by BbMep enhances NOD2 activation.

摘要

肠道细菌产生的可溶性肽聚糖片段是微生物群与宿主相互作用中的关键效应分子。在此，我们对BbMep进行了生化特性分析，BbMep是一种来自[具体来源未给出]的含NlpC/p60结构域的肽聚糖d，l-内肽酶，它能有效消化赖氨酸型或鸟氨酸型细胞壁肽聚糖。BbMep对人粪便来源的胞壁肽的消化增强了NOD2的激活。