Wang Yuqin, Zhao Yanhong, Chen Huan, Lu Tingting, Yang Rujie, Weng Xiuxiu, Li Wanhong
National Demonstration Center for Experimental Grassland Science Education, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Gansu Lanzhou 730020, China.
Tianzhu Animal Breeding Research Institute, Gansu Wuwei 733000, China.
Anim Biosci. 2024 Jun;37(6):1001-1006. doi: 10.5713/ab.23.0258. Epub 2024 Jan 20.
This study aimed to investigate the effect of Codonopsis pilosula polysaccharide (CPP) on the motility, mitochondrial integrity, acrosome integrity rate, and antioxidant ability of sheep sperm after preservation at 4°C.
Semen from healthy adult rams were collected and divided into four groups with separate addition of 0, 200, 400, and 1,000 mg/L CPP. Sperm motility was analyzed using the Computer-Assisted Semen Analysis software after preservation at 4°C for 24, 72, 120, and 168 h. Sperm acrosome integrity rate was analyzed by Giemsa staining at 24, 72, and 120 h, and mitochondrial membrane integrity was analyzed by Mito-Tracker Red CMXRos. The total antioxidant capacity (T-AOC) and malondialdehyde (MDA) content of spermatozoa were measured after 120 h of preservation.
The sperm viability and forward-moving sperm under 200 mg/L CPP were significantly higher than that in the control group at 72 h (61.28%±3.89% vs 52.83%± 0.70%, 51.53%±4.06% vs 42.84%±1.14%), and 168 h (47.21%±0.85% vs 41.43%±0.37%, 38.68%±0.87% vs 31.68%±0.89%). The percentage of fast-moving sperm (15.03%±1.10% vs 11.39%±1.03%) and slow-moving sperm (23.63%±0.76% vs 20.29%±1.11%) in the 200 mg/L group was significantly higher than control group at 168 h. The mitochondrial membrane integrity of the sperm in the group with 200 mg/L CPP was significantly higher than those in the control group after storage at 4°C for 120 h (74.76%±2.54% vs 65.67% ±4.51%, p<0.05). The acrosome integrity rate in the group with 200 mg/L (87.66%±1.26%) and 400 mg/L (84.00%±2.95%) was significantly higher than those in the control group (80.65%±0.16%) after storage for 24 h (p<0.05). CPP also increased T-AOC and decreased the MDA concentration after preservation at 4°C (p<0.05).
Adding CPP could improve the T-AOC of sperm, inhibit lipid peroxidation, and facilitate semen preservation.
本研究旨在探讨党参多糖（CPP）对4℃保存后的绵羊精子活力、线粒体完整性、顶体完整率及抗氧化能力的影响。
采集健康成年公羊的精液，分为四组，分别添加0、200、400和1000mg/L的CPP。在4℃保存24、72、120和168小时后，使用计算机辅助精液分析软件分析精子活力。在24、72和120小时通过吉姆萨染色分析精子顶体完整率，通过Mito-Tracker Red CMXRos分析线粒体膜完整性。保存120小时后测定精子的总抗氧化能力（T-AOC）和丙二醛（MDA）含量。
200mg/L CPP组在72小时（61.28%±3.89%对52.83%±0.70%，51.53%±4.06%对42.84%±1.14%）和168小时（47.21%±0.85%对41.43%±0.37%，38.68%±0.87%对31.68%±0.89%）的精子活力和向前运动精子显著高于对照组。200mg/L组在168小时的快速运动精子百分比（15.03%±1.10%对11.39%±1.03%）和慢速运动精子百分比（23.63%±0.76%对20.29%±1.11%）显著高于对照组。4℃保存120小时后，200mg/L CPP组精子的线粒体膜完整性显著高于对照组（74.76%±2.54%对65.67%±4.51%，p<0.05）。保存24小时后，200mg/L组（87.66%±1.26%）和400mg/L组（84.00%±2.95%）的顶体完整率显著高于对照组（80.65%±0.16%）（p<0.05）。CPP还增加了4℃保存后的T-AOC并降低了MDA浓度（p<0.05）。
添加CPP可提高精子的T-AOC，抑制脂质过氧化，促进精液保存。
