Possani L D, Martin B M, Svendsen I, Rode G S, Erickson B W
Biochem J. 1985 Aug 1;229(3):739-50. doi: 10.1042/bj2290739.
The complete primary structures of toxin II-14 from the Mexican scorpion Centruroides noxius Hoffmann and toxin gamma from the Brazilian scorpion Tityus serrulatus Lutz and Mello have been determined. Cleavage of toxin gamma after Met-6 with CNBr produced the 55-residue peptide 7-61, which maintained the four disulphide bonds but was not toxic to mice at a dose 3 times the lethal dose of native toxin gamma. Pairwise comparison by metric analysis of segment 1-50 of toxin gamma and the corresponding segments from two other South American scorpion toxins, five North American scorpion toxins, nine North African scorpion toxins and one Central Asian scorpion toxin showed that the three Brazilian toxins are intermediate between the North American and North African toxins. This result is consistent with the hypothesis that the South American and African continents were joined by a land connection in the distant past.
已确定了来自墨西哥蝎子Centruroides noxius Hoffmann的毒素II - 14以及来自巴西蝎子Tityus serrulatus Lutz和Mello的毒素γ的完整一级结构。用溴化氰在毒素γ的Met - 6之后进行切割，产生了55个残基的肽7 - 61，该肽保留了四个二硫键，但在剂量为天然毒素γ致死剂量3倍时对小鼠无毒。通过对毒素γ的1 - 50片段与另外两种南美蝎子毒素、五种北美蝎子毒素、九种北非蝎子毒素和一种中亚蝎子毒素的相应片段进行度量分析的成对比较表明，这三种巴西毒素介于北美毒素和北非毒素之间。这一结果与南美和非洲大陆在遥远过去通过陆地连接相连的假设一致。
