醛脱氢酶。一种在单一类型活性位点具有两种不同催化活性的酶。
Aldehyde dehydrogenase. An enzyme with two distinct catalytic activities at a single type of active site.
作者信息
Duncan R J
出版信息
Biochem J. 1985 Aug 15;230(1):261-7. doi: 10.1042/bj2300261.
Abstract
The evidence for and against the esterase and dehydrogenase active sites of aldehyde dehydrogenase being topologically distinct is examined. It is found that all the evidence (including all that previously amassed by others in favour of distinct binding domains) is actually consistent with, and in favour of, a single type of catalytic site having both activities. The existence of separate high-Km modulating sites for the enzyme is also questioned.
摘要
对醛脱氢酶酯酶和脱氢酶活性位点在拓扑结构上是否不同的支持和反对证据进行了研究。结果发现，所有证据（包括之前其他人收集的所有支持不同结合域的证据）实际上都与单一类型的具有两种活性的催化位点一致，并支持这一观点。同时也对该酶是否存在单独的高Km调节位点提出了质疑。
相似文献
1
Aldehyde dehydrogenase. An enzyme with two distinct catalytic activities at a single type of active site.醛脱氢酶。一种在单一类型活性位点具有两种不同催化活性的酶。
Biochem J. 1985 Aug 15;230(1):261-7. doi: 10.1042/bj2300261.
2
Evidence that the cytoplasmic aldehyde dehydrogenase-catalysed oxidation of aldehydes involves a different active-site group from that which catalyses the hydrolysis of 4-nitrophenyl acetate.有证据表明，细胞质醛脱氢酶催化的醛氧化反应所涉及的活性位点基团与催化4-硝基苯乙酸水解的活性位点基团不同。
Biochem J. 1988 Sep 15;254(3):903-6. doi: 10.1042/bj2540903.
3
Evidence that the slow conformation change controlling NADH release from the enzyme is rate-limiting during the oxidation of propionaldehyde by aldehyde dehydrogenase.有证据表明，在丙醛被醛脱氢酶氧化的过程中，控制NADH从该酶释放的缓慢构象变化是限速步骤。
Biochem J. 1987 Mar 15;242(3):803-8. doi: 10.1042/bj2420803.
4
Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.乙醛脱氢酶催化由乙酸对硝基苯酯和还原型辅酶I形成乙醛。
Biochem J. 1986 Sep 1;238(2):617-9. doi: 10.1042/bj2380617.
5
Reversal of part of the aldehyde dehydrogenase reaction pathway during the hydrolysis of an ester.酯水解过程中醛脱氢酶反应途径部分的逆转。
Biochem J. 1979 Nov 1;183(2):459-62. doi: 10.1042/bj1830459.
6
Evidence for two distinct active sites on aldehyde dehydrogenase.
J Biol Chem. 1988 Jan 25;263(3):1218-22.
7
A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase.硝基苯酯类与内酯类作为胞质醛脱氢酶底物的比较
Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj3160225.
8
Interaction of sheep liver cytosolic aldehyde dehydrogenase with quercetin, resveratrol and diethylstilbestrol.
Chem Biol Interact. 2001 Jan 30;130-132(1-3):57-69. doi: 10.1016/s0009-2797(00)00222-2.
9
Kinetic studies on the esterase activity of cytoplasmic sheep liver aldehyde dehydrogenase.细胞质绵羊肝脏醛脱氢酶酯酶活性的动力学研究
Biochem J. 1978 Jun 1;171(3):533-8. doi: 10.1042/bj1710533.
10
The action of cytoplasmic aldehyde dehydrogenase on methyl p-nitrophenyl carbonate and p-nitrophenyl dimethylcarbamate.细胞质醛脱氢酶对碳酸对硝基苯甲酯和二甲基氨基甲酸对硝基苯酯的作用。
Biochem J. 1989 Jan 15;257(2):579-84. doi: 10.1042/bj2570579.
引用本文的文献
1
Study of ALDH from -Expression, Purification and Characterisation of the Non-Substrate Specific, Thermophilic Enzyme Displaying Both Dehydrogenase and Esterase Activity.研究 ALDH 的表达、纯化和特性，该非底物特异性、嗜热酶同时具有脱氢酶和酯酶活性。
Cells. 2021 Dec 14;10(12):3535. doi: 10.3390/cells10123535.
2
Biochemical Characterization of Phenylacetaldehyde Dehydrogenases from Styrene-degrading Soil Bacteria.苯乙醛脱氢酶的生化特性研究来自于降解苯乙烯的土壤细菌。
Appl Biochem Biotechnol. 2021 Mar;193(3):650-667. doi: 10.1007/s12010-020-03421-8. Epub 2020 Oct 27.
3
Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate.以醋酸试卤灵为底物对胞质醛脱氢酶酯酶活性的研究。
Biochem J. 1997 Mar 15;322 ( Pt 3)(Pt 3):701-8. doi: 10.1042/bj3220701.
4
A comparison of nitrophenyl esters and lactones as substrates of cytosolic aldehyde dehydrogenase.硝基苯酯类与内酯类作为胞质醛脱氢酶底物的比较
Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj3160225.
5
Aldehyde dehydrogenase catalyses acetaldehyde formation from 4-nitrophenyl acetate and NADH.乙醛脱氢酶催化由乙酸对硝基苯酯和还原型辅酶I形成乙醛。
Biochem J. 1986 Sep 1;238(2):617-9. doi: 10.1042/bj2380617.
6
Inactivation of horse liver mitochondrial aldehyde dehydrogenase by disulfiram. Evidence that disulfiram is not an active-site-directed reagent.双硫仑对马肝线粒体醛脱氢酶的失活作用。双硫仑并非活性位点导向试剂的证据。
Biochem J. 1987 Mar 1;242(2):499-503. doi: 10.1042/bj2420499.
7
Effect of disulfiram on the pre-steady-state burst in the reactions of sheep liver cytoplasmic aldehyde dehydrogenase.双硫仑对绵羊肝脏细胞质醛脱氢酶反应中预稳态猝发的影响。
Biochem J. 1987 Dec 15;248(3):989-91. doi: 10.1042/bj2480989.
8
9
The action of cytoplasmic aldehyde dehydrogenase on methyl p-nitrophenyl carbonate and p-nitrophenyl dimethylcarbamate.细胞质醛脱氢酶对碳酸对硝基苯甲酯和二甲基氨基甲酸对硝基苯酯的作用。
Biochem J. 1989 Jan 15;257(2):579-84. doi: 10.1042/bj2570579.
10
Kinetics of p-nitrophenyl pivalate hydrolysis catalysed by cytoplasmic aldehyde dehydrogenase.细胞质醛脱氢酶催化对硝基苯基新戊酸酯水解的动力学
Biochem J. 1989 Jan 15;257(2):573-8. doi: 10.1042/bj2570573.
本文引用的文献
1
A reinvestigation of the purity, isoelectric points and some kinetic properties of the aldehyde dehydrogenases from sheep liver.对绵羊肝脏中醛脱氢酶的纯度、等电点及某些动力学性质的重新研究。
Eur J Biochem. 1981 Sep;119(1):79-84. doi: 10.1111/j.1432-1033.1981.tb05579.x.
2
The inactivation of aldehyde dehydrogenase by disulfiram in the presence of glutathione.在谷胱甘肽存在的情况下，双硫仑使乙醛脱氢酶失活。
Biochem J. 1981 Oct 1;199(1):255-8. doi: 10.1042/bj1990255.
3
The activation of aldehyde dehydrogenase by diethylstilboestrol and 2,2'-dithiodipyridine.己烯雌酚和2,2'-二硫代二吡啶对醛脱氢酶的激活作用。
Biochem J. 1982 Oct 1;207(1):81-9. doi: 10.1042/bj2070081.
4
5
Further studies of the action of disulfiram and 2,2'-dithiodipyridine on the dehydrogenase and esterase activities of sheep liver cytoplasmic aldehyde dehydrogenase.双硫仑和2,2'-二硫代二吡啶对绵羊肝脏细胞质醛脱氢酶的脱氢酶和酯酶活性作用的进一步研究
Biochem J. 1982 Jun 1;203(3):743-54. doi: 10.1042/bj2030743.
6
Kinetic properties of highly purified preparations of sheep liver cytoplasmic aldehyde dehydrogenase.绵羊肝脏细胞质醛脱氢酶高度纯化制剂的动力学特性
Biochem J. 1982 Jun 1;203(3):617-27. doi: 10.1042/bj2030617.
7
8
Relationship between the mechanisms of the esterase and dehydrogenase activities of the cytoplasmic aldehyde dehydrogenase from sheep liver. An alternative view.
Biochemistry. 1983 Aug 2;22(16):3784-91. doi: 10.1021/bi00285a011.
9
Hexose phosphate binding sites of fructose-6-phosphate,2-kinase:fructose-2,6-bisphosphatase. Interaction with N-bromoacetylethanolamine phosphate and 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphate.6-磷酸果糖-2-激酶：果糖-2,6-二磷酸酶的己糖磷酸结合位点。与N-溴乙酰乙醇胺磷酸和3-溴-1,4-二羟基-2-丁酮1,4-二磷酸的相互作用。
J Biol Chem. 1984 Nov 25;259(22):14023-8.
10
The purification and properties of the NAD-linked aldehyde dehydrogenase from pig brain.
Eur J Biochem. 1971 Sep 24;22(2):257-62. doi: 10.1111/j.1432-1033.1971.tb01539.x.
Zotero 插件