己烯雌酚和2,2'-二硫代二吡啶对醛脱氢酶的激活作用。
The activation of aldehyde dehydrogenase by diethylstilboestrol and 2,2'-dithiodipyridine.
作者信息
Kitson T M
出版信息
Biochem J. 1982 Oct 1;207(1):81-9. doi: 10.1042/bj2070081.
Abstract
- The activation of sheep liver cytoplasmic aldehyde dehydrogenase by diethylstilboestrol and by 2,2'-dithiodipyridine is described. The effects of the two modifiers are very similar with respect to variation with acetaldehyde concentration, pH and temperature. Thus the degree of activation is maximal when the enzyme is assayed at approx. 1 mM-acetaldehyde, is greater at 25 degrees C than at 37 degrees C, and is greater at pH 7.4 than at pH 9.75. With low concentrations of acetaldehyde both modifiers decrease the enzyme activity. 2. Diethylstilboestrol affects the sheep liver cytoplasmic enzyme in a very similar way to that previously described for a rabbit liver cytoplasmic enzyme. Preliminary experiments show that the same is true for a preparation of human liver aldehyde dehydrogenase. It is proposed that sensitivity to diethylstilboestrol (and steroids) is a common property of all mammalian cytoplasmic aldehyde dehydrogenases.
摘要
- 本文描述了己烯雌酚和2,2'-二硫代二吡啶对羊肝细胞质醛脱氢酶的激活作用。就乙醛浓度、pH值和温度变化而言，这两种调节剂的作用非常相似。因此，当酶在约1 mM乙醛浓度下进行测定时，激活程度最大；在25℃时比在37℃时更大；在pH 7.4时比在pH 9.75时更大。在低浓度乙醛条件下，两种调节剂都会降低酶活性。2. 己烯雌酚对羊肝细胞质酶的影响与先前描述的兔肝细胞质酶的影响非常相似。初步实验表明，人肝醛脱氢酶制剂也是如此。有人提出，对己烯雌酚（和类固醇）的敏感性是所有哺乳动物细胞质醛脱氢酶的共同特性。
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