蠲痹利节清方对高尿酸血症性痛风性关节炎大鼠模型治疗作用的机制研究
Mechanistic Study on the Therapeutic Effects of Juanbi Lijieqing Formula in a Rat Model of Gouty Arthritis with Hyperuricemia.
作者信息
Lu Yifan, Lu Chengyin, Jian Gonghui, Xiong Hui, Shu Yang
机构信息
Orthopedics Department, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410000, People's Republic of China.
Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, People's Republic of China.
出版信息
J Pain Res. 2025 Jun 9;18:2865-2877. doi: 10.2147/JPR.S517711. eCollection 2025.
BACKGROUND
This study evaluated the therapeutic effects and underlying mechanisms of Juanbi Lijieqing Formula (JBLJQF) in a rat model of gouty arthritis with hyperuricemia.
METHODS
Forty-two rats were randomly assigned to seven groups: control, model, etoricoxib, benzbromarone, and low-, medium-, and high-dose JBLJQF. General condition, joint swelling, behavioral performance, biochemical markers, and histopathology were assessed. Urate Anion Transporter 1(URAT1) expression was examined via immunofluorescence and Western blot.
RESULTS
JBLJQF improved general condition, reduced joint swelling, and alleviated kidney damage. Medium- and high-dose groups showed enhanced pain threshold and locomotor activity. Serum uric acid, TNF-α, and IL-1β levels were significantly decreased. URAT1 expression was downregulated, suggesting reduced renal urate reabsorption.
CONCLUSION
JBLJQF exerts anti-inflammatory, uric acid-lowering, and organ-protective effects in gouty arthritis with hyperuricemia, highlighting its potential for clinical application.
背景
本研究评估了蠲痹利节清方（JBLJQF）对高尿酸血症性痛风性关节炎大鼠模型的治疗效果及潜在机制。
方法
将42只大鼠随机分为7组：对照组、模型组、依托考昔组、苯溴马隆组以及低、中、高剂量JBLJQF组。评估大鼠的一般状况、关节肿胀程度、行为表现、生化指标及组织病理学变化。通过免疫荧光和蛋白质印迹法检测尿酸阴离子转运蛋白1（URAT1）的表达。
结果
JBLJQF改善了大鼠的一般状况，减轻了关节肿胀，并减轻了肾脏损伤。中、高剂量组大鼠的痛阈提高，运动活性增强。血清尿酸、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平显著降低。URAT1表达下调，提示肾脏尿酸重吸收减少。
结论
JBLJQF对高尿酸血症性痛风性关节炎具有抗炎、降尿酸及器官保护作用，凸显了其临床应用潜力。
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