Yang Yuhua, Yang Xianping, Xu Zhidong, Lu Qinhui, Zhang Qinghai
School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control Ministry of Education, Guizhou Medical University Guiyang China.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences Guiyang China.
Food Sci Nutr. 2025 Jun 13;13(6):e70391. doi: 10.1002/fsn3.70391. eCollection 2025 Jun.
Although zinc-fulvic acid chelate (Zn-FA) can efficiently reduce the cadmium (Cd) accumulation in rice grains, little is known about the impacts on other beneficial elements (such as zinc) levels in rice, along with the optimal concentration and underlying molecular mechanisms. The aim of this study is to investigate the effect of Zn-FA on Cd accumulation in rice and its metabolic mechanism through pot experiments. The results demonstrated that Zn-FA treatments significantly reduced Cd and increased Zn content in rice grains, with 10 g/L Zn-FA identified as the optimal concentration for spraying. Zn-FA treatment primarily reduced the translocation of Cd from stems to grains, thereby limiting Cd accumulation in the grain. Metabolomic analysis revealed that Zn-FA application significantly altered the metabolic profiles of rice leaves under Cd stress. In Yixiangyou586 (YXY586), Zn-FA involved the upregulation of flavonoids with antioxidant properties, along with associated pathways such as flavonoid biosynthesis, flavone, and flavonol biosynthesis; similarly, in Gengxiangyou703 (GXY703), Zn-FA modulated the metabolism of flavonoids. Under Zn-FA application, the reduction of Cd content and increase of Zn accumulation in rice grains are mainly related to the altered metabolism of phenolics, especially flavonoids in rice leaves. The findings of this study offer new insights into the mechanism of Zn-FA in reducing Cd content in rice, thereby providing a scientific foundation and technical support for ensuring safe crop production and promoting ecological safety.
尽管锌-富里酸螯合物（Zn-FA）能有效降低水稻籽粒中的镉（Cd）积累，但对于其对水稻中其他有益元素（如锌）含量的影响，以及最佳浓度和潜在分子机制却知之甚少。本研究旨在通过盆栽试验探究Zn-FA对水稻镉积累的影响及其代谢机制。结果表明，Zn-FA处理显著降低了水稻籽粒中的镉含量并提高了锌含量，其中10 g/L Zn-FA被确定为最佳喷施浓度。Zn-FA处理主要减少了镉从茎向籽粒的转运，从而限制了籽粒中的镉积累。代谢组学分析表明，在镉胁迫下，施用Zn-FA显著改变了水稻叶片的代谢谱。在宜香优586（YXY586）中，Zn-FA参与上调具有抗氧化特性的黄酮类化合物以及相关途径，如黄酮类生物合成、黄酮和黄酮醇生物合成；同样，在粳香优703（GXY703）中，Zn-FA调节了黄酮类化合物的代谢。在施用Zn-FA的情况下，水稻籽粒中镉含量的降低和锌积累的增加主要与酚类物质尤其是水稻叶片中黄酮类化合物代谢的改变有关。本研究结果为Zn-FA降低水稻镉含量的机制提供了新的见解，从而为确保作物安全生产和促进生态安全提供科学依据和技术支持。
