Ruttink Tom, Connolly Bryan, Lamour Kurt, Hulvey Jonathan, Bawin Yves, Hilley Erin C, Tandy Peter
Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Melle, Belgium.
Department of Plant Biotechnology and Bioinformatics, Faculty of Sciences, Ghent University, Ghent, Belgium.
PLoS One. 2025 Jun 16;20(6):e0325657. doi: 10.1371/journal.pone.0325657. eCollection 2025.
Triosteum (Caprifoliaceae) is a genus of herbaceous perennial angiosperms composed of three Asian and three North American species. The range of two similar species, T. aurantiacum and T. perfoliatum, overlap in the state of Massachusetts, USA, where the latter species is considered locally endangered and protected by regulations. The population occurring in Barnstable Co. MA, USA was morphologically atypical and had intermediate characteristics between the two taxa. Genome-wide molecular fingerprinting was used to identify this population by comparison to nearby populations with typical morphology for each of the reference species T. aurantiacum and T. perfoliatum. A set of 220,518 high quality SNPs were used to calculate expected heterozygosity, FIS, nucleotide diversity, and FST, and to create short multi-allelic haplotype markers. Detailed population characterization was performed using Principal Component Analysis (PCA) and fastSTRUCTURE analysis on SNPs, and the haplotype markers were used to create a NJ phylogenetic tree, and pairwise comparisons of the genetic distance (Jaccard Inverse Distance) between individual plants within and between subpopulations, populations, and species. Furthermore, complete chloroplast genome sequences were created, and structural polymorphisms characterized and compared to a range of closely related species. Taken together, the data reveals a fine subpopulation structure within the morphologically atypical population at Barnstable Co. MA, that are more closely related to T. perfoliatum than to T. aurantiacum.
刺续断属（忍冬科）是一类多年生草本被子植物，由三个亚洲物种和三个北美物种组成。两种相似的物种，金色刺续断（Triosteum aurantiacum）和贯穿叶刺续断（Triosteum perfoliatum），在美国马萨诸塞州的分布范围有重叠，后者在当地被视为濒危物种并受到法规保护。美国马萨诸塞州巴恩斯特布尔县出现的种群在形态上不典型，具有这两个分类群之间的中间特征。通过与金色刺续断和贯穿叶刺续断这两个参考物种具有典型形态的附近种群进行比较，利用全基因组分子指纹识别该种群。一组220,518个高质量单核苷酸多态性（SNP）用于计算预期杂合度、近交系数（FIS）、核苷酸多样性和固定指数（FST），并创建短的多等位基因单倍型标记。使用主成分分析（PCA）和对SNP的快速结构分析进行详细的种群特征描述，单倍型标记用于构建邻接系统发育树，并对亚种群内和亚种群之间、种群之间以及物种之间的个体植物的遗传距离（杰卡德逆距离）进行成对比较。此外，还创建了完整的叶绿体基因组序列，对结构多态性进行了表征，并与一系列近缘物种进行了比较。综合来看，数据揭示了美国马萨诸塞州巴恩斯特布尔县形态不典型种群内的精细亚种群结构，这些亚种群与贯穿叶刺续断的关系比与金色刺续断的关系更密切。
