Botany Department, University of Wisconsin-Madison, 53706, USA.
Ann Bot. 2012 Dec;110(8):1623-9. doi: 10.1093/aob/mcs222. Epub 2012 Oct 24.
The amount of DNA in an unreplicated haploid nuclear genome (C-value) ranges over several orders of magnitude among plant species and represents a key metric for comparing plant genomes. To extend previously published datasets on plant nuclear content and to characterize the DNA content of many species present in one region of North America, flow cytometry was used to estimate C-values of woody and herbaceous species collected in Wisconsin and the Upper Peninsula of Michigan, USA.
A total of 674 samples and vouchers were collected from locations across Wisconsin and Michigan, USA. From these, C-value estimates were obtained for 514 species, subspecies and varieties of vascular plants. Nuclei were extracted from samples of these species in one of two buffers, stained with the fluorochrome propidium iodide, and an Accuri C-6 flow cytometer was used to measure fluorescence peaks relative to those of an internal standard. Replicate extractions, coefficients of variation and comparisons to published C-values in the same and related species were used to confirm the accuracy and reliability of our results.
Prime C-values for 407 taxa are provided for which no published data exist, including 390 angiosperms, two gymnosperms, ten monilophytes and five lycophytes. Non-prime reports for 107 additional taxa are also provided. The prime values represent new reports for 129 genera and five families (of 303 genera and 97 families sampled). New family C-value maxima or minima are reported for Betulaceae, Ericaceae, Ranunculaceae and Sapindaceae. These data provide the basis for phylogenetic analyses of C-value variation and future analyses of how C-values covary with other functional traits.
未复制的单倍体核基因组（C 值）中的 DNA 量在植物物种之间跨越几个数量级，是比较植物基因组的关键指标。为了扩展之前发表的关于植物核含量的数据，并描述北美一个地区存在的许多物种的 DNA 含量，我们使用流式细胞术来估计在美国威斯康星州和密歇根州上半岛采集的木本和草本物种的 C 值。
从美国威斯康星州和密歇根州的多个地点共采集了 674 个样本和凭证。在此基础上，我们获得了 514 种维管植物的种、亚种和变种的 C 值估计值。从这些物种的样本中提取核，用荧光染料碘化丙啶染色，并使用 Accuri C-6 流式细胞仪测量相对于内部标准的荧光峰。重复提取、变异系数以及与同一物种和相关物种中已发表的 C 值的比较，用于确认我们结果的准确性和可靠性。
提供了 407 个分类群的主要 C 值，其中包括 390 种被子植物、2 种裸子植物、10 种单叶植物和 5 种石松植物，这些分类群没有发表的数据。还提供了 107 个额外分类群的非主要报告。这些主要值代表了 129 个属和 5 个科（在 303 个属和 97 个科中取样）的新报告。报道了桦木科、石楠科、毛茛科和无患子科的新的科 C 值最大值或最小值。这些数据为 C 值变异的系统发育分析和未来分析 C 值与其他功能特征的相关性提供了基础。
