Konyshev Ilya, Dudina Lyubov, Belozerov Vladislav, Ivanov Sergey, Dentovskaya Svetlana, Anisimov Andrey, Byvalov Andrey
Department of Biotechnology, Federal State Budgetary Educational Institute of Higher Education Vyatka State University, Kirov, Russia.
Laboratory of Microbial Physiology, Institute of Physiology, Komi Science Center, Federal Research Center Komi Science Center, Ural Branch, Russian Academy of Sciences, Syktyvkar, Russia.
Eur Biophys J. 2025 Jun 16. doi: 10.1007/s00249-025-01768-6.
There has been a great interest in developing the phage-containing remedy against plague caused by antimicrobial resistant strains of Yersinia pestis, which have been increasingly isolated in recent years from sick humans and animals. Studies thus are under way to develop a "phage cocktail", which is expected to be effective against a wide range of pathogenic strains. Our paper sheds light on the role of Y. pestis antigen PsaA in reception of the phage L-413C, which might be a possible component of such a "cocktail". Using optical trapping (OT) and atomic force microscopy (AFM), we showed that PsaA-positive cells and PsaA-coated beads or cantilevers bound more effectively to a substrate coated with L-413C rather than Pokrovskaya phage. Comparing two isogenic strains of Y. pestis (EV and EV∆psaA), we found that when bacteria and phages are co-incubated under slightly acidic pH, as if in a eukaryotic cell, PsaA-positive cells bound the phage L-413C more effectively. There is good evidence to say that L-413C may become a component of a new anti-plague therapy due to its high ability to interact with the pili-forming protein PsaA from the outer membrane of Y. pestis.
近年来，从患病的人类和动物身上越来越多地分离出对抗菌素耐药的鼠疫耶尔森菌菌株，因此人们对开发含噬菌体的鼠疫治疗方法产生了浓厚兴趣。目前正在开展研究以开发一种“噬菌体鸡尾酒”，预计它能有效对抗多种致病菌株。我们的论文揭示了鼠疫耶尔森菌抗原PsaA在噬菌体L-413C吸附中的作用，而L-413C可能是这种“鸡尾酒”的一种可能成分。利用光镊（OT）和原子力显微镜（AFM），我们发现PsaA阳性细胞以及包被有PsaA的珠子或悬臂与包被有L-413C的底物结合比与波克罗夫斯卡亚噬菌体结合更有效。比较鼠疫耶尔森菌的两个同基因菌株（EV和EV∆psaA），我们发现当细菌和噬菌体在略酸性pH条件下共同孵育时，就如同在真核细胞中一样，PsaA阳性细胞能更有效地结合噬菌体L-413C。有充分证据表明，由于L-413C与鼠疫耶尔森菌外膜中形成菌毛的蛋白PsaA具有很高的相互作用能力，可以成为一种新型抗鼠疫疗法的组成部分。
