Institute of Physiology of Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Komi Republic 167982, Russian Federation; Vyatka State University, Kirov 610000, Russian Federation.
Institute of Physiology of Кomi Science Centre of the Ural Branch of the Russian Academy of Sciences, FRC Komi SC UB RAS, Syktyvkar, Komi Republic 167982, Russian Federation; Vyatka State University, Kirov 610000, Russian Federation.
J Biomech. 2020 Jan 23;99:109504. doi: 10.1016/j.jbiomech.2019.109504. Epub 2019 Nov 13.
This article reports the force spectroscopy investigation of interactions between lipopolysaccharides (LPSs) of two species from Yersinia genus and complementary (or heterologous) monoclonal antibodies (mAbs). We have obtained the experimental data by optical trapping on the "sensitized polystyrene microsphere - sensitized glass substrate" model system at its approach - retraction in vertical plane. We detected non-specific interactions in low-amplitude areas on histograms mainly due to physicochemical properties of abiotic surface and specific interactions in complementary pairs "antigen - antibodies" in high-amplitude areas (100-120 pN) on histograms. The developed measurement procedure can be used for detection of rupture forces in other molecular pairs.
本文报道了脂多糖（LPS）两种来自耶尔森氏菌属物种与互补（或异源）单克隆抗体（mAb）之间相互作用的力谱研究。我们通过在垂直平面上进样-退样的“敏化聚苯乙烯微球-敏化玻璃基底”模型系统，用光镊获得了实验数据。我们在直方图的低幅度区域检测到非特异性相互作用，这主要归因于非生物表面的物理化学性质，在直方图的高幅度区域（100-120 pN）检测到互补对“抗原-抗体”的特异性相互作用。开发的测量程序可用于检测其他分子对的断裂力。
