Chukwunyere Ugochukwu, Sayıner Serkan, Mercan Merve, Çetinel Şule, Çaliş İhsan, Sehirli Ahmet Özer
Department of Pharmacology, Faculty of Pharmacy, Near East University, 99138, Nicosia, North Cyprus.
Department of Biochemistry, Faculty of Veterinary Medicine, Near East University, 99138, Nicosia, North Cyprus.
Med Oncol. 2025 Jun 16;42(7):267. doi: 10.1007/s12032-025-02823-0.
This study investigated the protective effects of verbascoside (VER) against 5-fluorouracil (5-FU)-induced gastrointestinal mucositis in Wistar albino rats.
The study involved 30 female rats that were equally divided into five groups as follows: Control group, 5-FU group (400 mg/kg, IP), VER-only group (0.2 mg/kg, IP), 5-FU (400 mg/kg, IP) + VER (0.2 mg/kg, IP) group, and 5-FU (400 mg/kg, IP) + VER (0.4 mg/kg, IP) group. All animals were euthanized four days after 5-FU administration. Gastrointestinal tissues (esophagus, stomach, duodenum, jejunum, ileum, and colon) and blood sera were collected for histopathological and biochemical analyses. Tissue and sera analyses showed that 5-FU caused significant alterations marked by increases in matrix metalloproteinases (MMP-1, -2, -8), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) levels and decreases in tissue inhibitor of metalloproteinases-1 (TIMP-1), albumin, and total protein levels. VER treatment effectively attenuated these 5-FU-induced changes, with trends toward improved histological outcomes at higher doses.
The findings strongly suggest that VER offers significant protection, and these results warrant further investigation into its potential clinical application as an adjunct therapy to mitigate gastrointestinal and other toxicities associated with 5-FU chemotherapy.
本研究调查了毛蕊花糖苷（VER）对5-氟尿嘧啶（5-FU）诱导的Wistar白化大鼠胃肠道粘膜炎的保护作用。
本研究涉及30只雌性大鼠，将其平均分为五组，如下：对照组、5-FU组（400mg/kg，腹腔注射）、单纯VER组（0.2mg/kg，腹腔注射）、5-FU（400mg/kg，腹腔注射）+VER（0.2mg/kg，腹腔注射）组和5-FU（400mg/kg，腹腔注射）+VER（0.4mg/kg，腹腔注射）组。在给予5-FU四天后，所有动物均被安乐死。收集胃肠道组织（食管、胃、十二指肠、空肠、回肠和结肠）和血清进行组织病理学和生化分析。组织和血清分析表明，5-FU引起了显著变化，表现为基质金属蛋白酶（MMP-1、-2、-8）、碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）水平升高，金属蛋白酶组织抑制剂-1（TIMP-1）、白蛋白和总蛋白水平降低。VER治疗有效地减轻了这些5-FU诱导的变化，在较高剂量下有组织学结果改善的趋势。
研究结果强烈表明，VER具有显著的保护作用，这些结果值得进一步研究其作为辅助治疗减轻与5-FU化疗相关的胃肠道和其他毒性的潜在临床应用。
