毛蕊花糖苷对MCF-7和MDA-MB-231细胞的细胞毒性作用
Cytotoxic Effects of Verbascoside on MCF-7 and MDA-MB-231.
作者信息
Şenol Hülya, Tulay Pınar, Ergören Mahmut Çerkez, Hanoğlu Azmi, Çalış İhsan, Mocan Gamze
机构信息
Near East University Faculty of Medicine, Department of Medical Biology, Nicosia, North Cyprus
Near East University Faculty of Medicine, Department of Medical Genetics, Nicosia, North Cyprus
出版信息
Turk J Pharm Sci. 2021 Oct 28;18(5):637-644. doi: 10.4274/tjps.galenos.2021.36599.
OBJECTIVES
Verbascoside, also known as acteoside/kusaginin, has attracted a great attention due to its pharmacological features. In this study, we aimed to determine the cytotoxic effects of pure verbascoside isolated from L. plant in both MCF-7 and MDA-MB-231 cell lines .
MATERIALS AND METHODS
MCF-7 and MDA-MB 231 cells were treated with verbascoside (100, 48, 25, 10, 1, 0.5, and 0.1 μM) for 24, 48, and 72 hours. Cytotoxic effect of verbascoside in MCF-7 and MDA-MB-231 cells was assessed using TEBU-BIO cell counting kit 8.
RESULTS AND CONCLUSION
IC values for 24, 48, and 72 h verbascoside exposure of MCF-7 cells were determined as 0.127, 0.2174, and 0.2828 μM, respectively. R values were calculated as 0.9630, 0.8789 and 0.8752, respectively. Two-Way ANOVA multiple comparison test results showed that 100 μM verbascoside has the highest cytotoxic effect on MCF-7 breast cancer (BC) cells after 72 h of exposure. IC values for 24, 48 and 72 h verbascoside exposure of MDA-MB 231 cells were determined as 0.1597, 0.2584 and 0.2563 μM, respectively and R values were calculated as 0.8438, 0.5107 and 0.9203, respectively. Two-Way ANOVA multiple comparisons test results showed that 100 μM verbascoside has the highest cytotoxic effect on MDA-MB 231 BC cells after 24, 48 and 72 h of exposure.
目的
毛蕊花糖苷，也称为洋丁香酚苷/库萨金宁，因其药理特性而备受关注。在本研究中，我们旨在确定从某植物中分离出的纯毛蕊花糖苷对MCF-7和MDA-MB-231细胞系的细胞毒性作用。
材料与方法
用毛蕊花糖苷（100、48、25、10、1、0.5和0.1μM）处理MCF-7和MDA-MB 231细胞24、48和72小时。使用TEBU-BIO细胞计数试剂盒8评估毛蕊花糖苷对MCF-7和MDA-MB-231细胞的细胞毒性作用。
结果与结论
MCF-7细胞在暴露于毛蕊花糖苷24、48和72小时后的半数抑制浓度（IC）值分别确定为0.127、0.2174和0.2828μM。相关系数（R值）分别计算为0.9630、0.8789和0.8752。双向方差分析多重比较测试结果表明，在暴露72小时后，100μM毛蕊花糖苷对MCF-7乳腺癌（BC）细胞具有最高的细胞毒性作用。MDA-MB 231细胞在暴露于毛蕊花糖苷24、48和72小时后的IC值分别确定为0.1597 μM、0.2584 μM和0.2563 μM，R值分别计算为0.8438、0.5107和0.9203。双向方差分析多重比较测试结果表明，在暴露24、48和72小时后，100μM毛蕊花糖苷对MDA-MB 231 BC细胞具有最高的细胞毒性作用。
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