Okumura T, Nakayama R, Sago T, Saito K
Biochim Biophys Acta. 1985 Nov 14;837(2):197-207.
Primary cultured rat hepatocytes were shown to bind to prostaglandins E1, E2, D2 and F2 alpha and then rapidly degrade at 37 degrees C. 6-Ketoprostaglandin F1 alpha and thromboxane B2, which are inactive metabolites of prostaglandin I2 and thromboxane A2, respectively, bound less effectively to the cells and were not degraded. Incubation of hepatocytes with 3H-labeled prostaglandins, treatment of the cells at an acidic pH, and analysis of the acid solution by thin-layer chromatography, showed that the radioactive material was bound to the cell surface and consisted of intact prostaglandin and its metabolites. The metabolites of prostaglandin E that accumulated in the culture medium were purified by silicic acid column and silica gel thin-layer chromatographies, and analyzed by gas chromatography-mass spectrometry. Prostaglandins E1 and E2 gave exactly the same metabolites, which were identified as dinorprostaglandin E1 and tetranorprostaglandin E1, representing products of beta-oxidation. These data suggest that part of the carboxyl side chain of prostaglandins, but not of inactive metabolites, was eliminated by a beta-oxidation system in the hepatocytes, while the rest of the molecule was not degraded appreciably and was rapidly transferred to the outside of the cells.
原代培养的大鼠肝细胞被证明能与前列腺素E1、E2、D2和F2α结合，然后在37℃迅速降解。6-酮前列腺素F1α和血栓素B2分别是前列腺素I2和血栓素A2的无活性代谢产物，它们与细胞的结合效率较低且不会被降解。用3H标记的前列腺素孵育肝细胞，在酸性pH下处理细胞，并通过薄层色谱分析酸性溶液，结果表明放射性物质与细胞表面结合，且由完整的前列腺素及其代谢产物组成。通过硅酸柱和硅胶薄层色谱法对培养基中积累的前列腺素E的代谢产物进行纯化，并通过气相色谱-质谱联用仪进行分析。前列腺素E1和E2产生完全相同的代谢产物，这些代谢产物被鉴定为二去甲前列腺素E1和四去甲前列腺素E1，代表β-氧化产物。这些数据表明，前列腺素羧基侧链的一部分（而非无活性代谢产物的羧基侧链）在肝细胞中通过β-氧化系统被消除，而分子的其余部分没有明显降解，并迅速转移到细胞外。
