Karaseva L I, Pankov Iu A
Biokhimiia. 1985 Sep;50(9):1528-34.
Two CNBr fragments of sea whale prolactin containing 69 and 41 amino acid residues, respectively, were hydrolyzed by trypsin, and the hydrolytic products were separated by the paper peptide mapping technique. The amino acid sequence of 17 homogeneous peptides was studied by the Edman method as well as by hydrolysis with carboxypeptidases A and B. Based on the experimental data and the previously published results the primary structure of sea whale prolactin made up of 199 amino acid residues was proposed.
分别含有69个和41个氨基酸残基的海鲸催乳素的两个溴化氰片段用胰蛋白酶水解，水解产物通过纸肽图谱技术进行分离。用埃德曼法以及用羧肽酶A和B水解研究了17个均一肽的氨基酸序列。根据实验数据和先前发表的结果，提出了由199个氨基酸残基组成的海鲸催乳素的一级结构。
