F Jancy, D Gopi
Department of Chemistry, Periyar University, Salem 636011, Tamil Nadu, India.
Department of Chemistry, Periyar University, Salem 636011, Tamil Nadu, India..
Int J Biol Macromol. 2025 Jul;318(Pt 4):145267. doi: 10.1016/j.ijbiomac.2025.145267. Epub 2025 Jun 15.
The hydroxyapatite (HAp) with the chemical formula Ca₁₀(PO₄)₆(OH)₂ is derived from the eggshells as a calcium source through the co-precipitation method, has been repurposed into valuable biomaterials with a strong affinity for ion substitution, notably the Sm. Furthermore, the Acacia nilotica gum was utilized for the preparation of a composite, which is excellent in water solubility and composed of both amorphous (96.2 %) and crystalline (3.8 %) powder forms. The present biogenic synthesis method consists the eggshells-based Sm-HAp/Acacia nilotica gum-based polymer composite by the co-precipitation method, and the as synthesized materials was characterized by the Fourier Transform Infrared Spectroscopy (FTIR), the X-ray Diffraction (XRD), the Scanning Electron Microscopy (SEM), and the Transmission Electron Microscopy (TEM). The antibacterial efficiency of the composite was assessed using the Gram-negative bacteria Escherichia coli (E. coli) and the Gram-positive bacteria Staphylococcus aureus (S. aureus). This study investigated the antioxidant activity of the Sm-HAp/ANG composite by analyzing its DPPH activity. The biocompatibility of the HOS MG-63 cells on the Sm-HAp/Acacia nilotica gum composite was also evaluated by the MTT assay test and bioactivity studies. The swelling and degradation behaviors of the newly developed materials were also assessed in a simulated body fluid (SBF) solution. The obtained results indicate that the synthesized eggshells-based Sm-HAp/Acacia nilotica gum-based polymer biocomposite possesses effective antibacterial and biocompatible properties.
化学式为Ca₁₀(PO₄)₆(OH)₂的羟基磷灰石（HAp）是通过共沉淀法从蛋壳中作为钙源衍生而来的，已被重新用作对离子取代具有强亲和力的有价值生物材料，特别是对钐（Sm）。此外，阿拉伯胶被用于制备一种复合材料，该复合材料具有优异的水溶性，由无定形（96.2%）和结晶（3.8%）粉末形式组成。目前的生物合成方法是通过共沉淀法制备基于蛋壳的Sm-HAp/阿拉伯胶基聚合物复合材料，并用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对合成材料进行表征。使用革兰氏阴性菌大肠杆菌（E. coli）和革兰氏阳性菌金黄色葡萄球菌（S. aureus）评估该复合材料的抗菌效率。本研究通过分析其DPPH活性来研究Sm-HAp/ANG复合材料的抗氧化活性。还通过MTT试验和生物活性研究评估了HOS MG-63细胞在Sm-HAp/阿拉伯胶复合材料上的生物相容性。在模拟体液（SBF）溶液中也评估了新开发材料的溶胀和降解行为。所得结果表明，合成的基于蛋壳的Sm-HAp/阿拉伯胶基聚合物生物复合材料具有有效的抗菌和生物相容性特性。
