Goswami Rakesh, Sarkar Aniket, Bandyopadhyay Bidyut, Sadhukhan Sanjoy
Department of Biotechnology, Oriental Institute of Science and Technology, Vidyasagar University, Midnapore, West Bengal, India.
Plant Molecular Biology Laboratory, Department of Botany, Raiganj University, Uttar Dinajpur, Raiganj, West Bengal, 733134, India.
Mol Biol Rep. 2025 Jun 17;52(1):608. doi: 10.1007/s11033-025-10690-1.
Hot springs are natural geothermal environments that harbour thermophilic microorganisms with significant biotechnological potential. Despite extensive studies on many Indian hot springs, Kharasinpur Hot Spring in West Bengal remains totally unexplored in terms of microbial diversity and physicochemical properties.
A total of seven bacterial isolates were obtained from water samples collected at the Kharasinpur Hot Spring. These isolates underwent morphological, physiological, biochemical, and molecular characterization through 16S rRNA gene sequencing. Water samples were analyzed for physicochemical parameters such as temperature, pH, total hardness, iron concentration, salt content, and total dissolved solids (TDS).
The water temperature was recorded at 65 °C with a slightly alkaline pH (7.24). The 16S rRNA analysis identified isolates belonging to the phyla Pseudomonadota (e.g., Pseudomonas sp., Hydrogenophaga sp.) and Bacillota (e.g., Staphylococcus sp., Neobacillus sp.). The high iron content in the hot spring water was notable and rendered it unsafe for direct human consumption.
This study provides novel insights into the microbial diversity and physicochemical characteristics of a relatively unstudied Indian hot spring. The findings contribute to broader research efforts on hot spring ecosystems in India, enhancing our understanding of their microbial ecology and potential health implications.
温泉是自然地热环境，其中蕴藏着具有重要生物技术潜力的嗜热微生物。尽管对许多印度温泉进行了广泛研究，但西孟加拉邦的哈拉辛布尔温泉在微生物多样性和理化性质方面仍完全未被探索。
从哈拉辛布尔温泉采集的水样中总共获得了七株细菌分离株。这些分离株通过16S rRNA基因测序进行了形态、生理、生化和分子特征分析。对水样进行了温度、pH值、总硬度、铁浓度、盐含量和总溶解固体（TDS）等理化参数分析。
记录的水温为65°C，pH值略呈碱性（7.24）。16S rRNA分析确定分离株属于假单胞菌门（例如假单胞菌属、嗜氢菌属）和芽孢杆菌门（例如葡萄球菌属、新芽孢杆菌属）。温泉水中的高铁含量值得注意，使其不适合人类直接饮用。
本研究为一个相对未被研究的印度温泉的微生物多样性和理化特征提供了新的见解。这些发现有助于印度温泉生态系统的更广泛研究工作，增进我们对其微生物生态学及其潜在健康影响的理解。
