Li Wei-Jun, Hu Kai-Ping, Zhong Xin, Song Shui-Lin, Xu Cui-Kang, Xie Qing-Xiu, Li Xiao-Zhen
Department of Plant Protection, College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
Front Microbiol. 2025 Jun 2;16:1567154. doi: 10.3389/fmicb.2025.1567154. eCollection 2025.
Different insect tissues represent heterogeneous niches with distinct physiological and biochemical characteristics, and therefore host different bacterial communities.
In this study, those overlapping and specific bacterial communities in the female gut (fG), male gut (mG), female reproductive system (fR), and male reproductive system (mR) of (Hendel) adults were determined by high-throughput sequencing targeting 16S rRNA gene.
The richness of bacterial taxa based on OTU was higher in fR compared to the other three tissues. Among the 29 identified bacterial phyla, Pseudomonadota, Bacillota, and Bacteroidota were predominant, while among the 48 identified genera, , , , , and were common in the four tissues. fG harbored specific bacterial genus , mG harbored specific bacterial genera , , , , and , fR harbored specific bacterial genera , and , and mR harbored specific bacterial genera , , , , , and . In the 35 annotated KEGG pathways, high-abundance bacterial taxa were mainly enriched in these pathways of membrane transport, carbohydrate metabolism, amino acid metabolism, replication and repair, and energy metabolism, while low-abundance bacterial taxa were involved in these pathways of cardiovascular diseases, circulatory system, and excretory system. The abundances of the 5 pathways associated with cardiovascular diseases, circulatory system, excretory system, membrane transport, and polysaccharide biosynthesis and metabolism exhibited greater variations among fG, mG, fR, and mR. Among them, the two pathways abundances of cardiovascular disease and circulatory system were higher in the reproductive system, whereas the other three pathways abundances were higher in the female gut.
Our study revealed the abundance, composition and function of overlapping and specific bacterial communities in the gut and reproductive system of , providing valuable information for inhibiting the occurrence of by interfering with these functional bacterial communities in tissues.
不同昆虫组织代表具有不同生理和生化特征的异质生态位，因此宿主不同的细菌群落。
在本研究中，通过靶向16S rRNA基因的高通量测序，确定了（亨德尔）成虫雌性肠道（fG）、雄性肠道（mG）、雌性生殖系统（fR）和雄性生殖系统（mR）中的重叠和特定细菌群落。
基于OTU的细菌分类群丰富度在fR中高于其他三个组织。在29个已鉴定的细菌门中，假单胞菌门、芽孢杆菌门和拟杆菌门占主导地位，而在48个已鉴定的属中， 、 、 、 、 和 在四个组织中很常见。fG中含有特定的细菌属 ，mG中含有特定的细菌属 、 、 、 、 和 ，fR中含有特定的细菌属 、 和 ，mR中含有特定的细菌属 、 、 、 、 和 。在35条注释的KEGG途径中，高丰度细菌分类群主要富集在膜运输、碳水化合物代谢、氨基酸代谢、复制和修复以及能量代谢等途径中，而低丰度细菌分类群参与心血管疾病、循环系统和排泄系统等途径。与心血管疾病、循环系统、排泄系统、膜运输以及多糖生物合成和代谢相关的5条途径的丰度在fG、mG、fR和mR之间表现出更大的差异。其中，心血管疾病和循环系统这两条途径的丰度在生殖系统中较高，而其他三条途径的丰度在雌性肠道中较高。
我们的研究揭示了 肠道和生殖系统中重叠和特定细菌群落的丰度、组成和功能，为通过干扰组织中的这些功能细菌群落来抑制 的发生提供了有价值的信息。
