Yin Peng-Kai, Xiao Huai, Yang Zhi-Bin, Yang Da-Song, Yang Yin-He
Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, China.
College of Pharmacy, Dali University, Dali, China.
Front Microbiol. 2024 Mar 11;15:1288051. doi: 10.3389/fmicb.2024.1288051. eCollection 2024.
Wasps play important roles as predators and pollinators in the ecosystem. The Jingpo minority residing in Yunnan Province, China, has a traditional practice of using wine infused with mature wasps as a customary remedy for managing rheumatoid arthritis. The larva of the wasp is also a tasteful folk dish that has created a tremendous market. There is a paucity of survival knowledge, which has greatly restricted their potential applications in food and healthcare. Recent research has highlighted the importance of gut microbiota in insect growth. Nevertheless, there is still a lack of understanding regarding the composition, changes, and functions of the gut microbiota in during development. In this research, the gut microbiota were investigated across three growth stages of using a metagenomic technology. The result revealed that there are significant variations in the proportion of main gut microbes during the metamorphosis of . Tenericutes were found to dominate during the larval stage, while Proteobacteria emerged as the dominant group post-pupation. Through a comprehensive analysis of the gut microbiota metagenome, this study revealed functional differences in the wasp gut microbiota at various growth stages. During the larval stage, the gut microbiota plays a central role in promoting metabolism. Following pupation, the gut microbiota exhibited diversified functions, likely due to the complex environments and diverse food sources encountered after metamorphosis. These functions included amino acid metabolism, compound degradation, and defense mechanisms. This research provides an extensive dataset on the gut microbiota during the metamorphosis of , contributing to a deeper understanding of the influence of gut microbiota on wasp growth. Furthermore, this study uncovers a unique microbial treasure within insect guts, which is important for advancing the application of wasps in the fields of food and medicine.
黄蜂在生态系统中作为捕食者和传粉者发挥着重要作用。居住在中国云南省的景颇族有将成熟黄蜂泡的酒用作治疗类风湿性关节炎传统疗法的习俗。黄蜂幼虫也是一道美味的民间菜肴，创造了巨大的市场。目前关于黄蜂生存的知识匮乏，这极大地限制了它们在食品和医疗保健方面的潜在应用。最近的研究强调了肠道微生物群在昆虫生长中的重要性。然而，对于黄蜂发育过程中肠道微生物群的组成、变化及其功能仍缺乏了解。在本研究中，利用宏基因组技术对黄蜂三个生长阶段的肠道微生物群进行了调查。结果显示，在黄蜂变态发育过程中，主要肠道微生物的比例存在显著差异。柔膜菌门在幼虫阶段占主导地位，而变形菌门在化蛹后成为优势菌群。通过对肠道微生物群宏基因组的综合分析，本研究揭示了黄蜂在不同生长阶段肠道微生物群的功能差异。在幼虫阶段，肠道微生物群在促进新陈代谢方面发挥着核心作用。化蛹后，肠道微生物群表现出多样化的功能，这可能是由于变态发育后遇到的复杂环境和多样食物来源所致。这些功能包括氨基酸代谢、化合物降解和防御机制。本研究提供了关于黄蜂变态发育过程中肠道微生物群的大量数据集，有助于更深入地了解肠道微生物群对黄蜂生长的影响。此外，本研究发现了昆虫肠道内独特的微生物宝藏，这对于推动黄蜂在食品和医药领域的应用具有重要意义。
