Li Zhihao, Xu Hui, Chen Hao, Chen Liang, Zang Hong, Qian Yi
General Surgery Department, The Second Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Pathology Department, The Second Affiliated Hospital of Nantong University, Nantong, China.
Transl Cancer Res. 2025 May 30;14(5):3175-3185. doi: 10.21037/tcr-2025-812. Epub 2025 May 27.
Long noncoding RNA (lncRNA) TEX41 has been linked to the progression of various kinds of cancers. In this study, the expression of lncRNA TEX41 and cell biological function of lncRNA TEX41 were examined in the context of hepatocellular carcinoma (HCC) to measure their ability to predict HCC progression. The relationship between lncRNA TEX41, miR-200a-3p, and potential downstream target gene was further explored. The purpose of the present study was to provide a new theoretical basis for the diagnosis and treatment of HCC.
The GEPIA2, starBase and Gene Expression Omnibus (GEO) databases were used to analyze the biological functions and molecular mechanisms of lncRNA TEX41. The expression of lncRNA TEX41 in clinical tissue samples and cell lines was detected via quantitative real-time polymerase chain reaction (qRT-PCR). We investigated the impact of TEX41 on the proliferation, migration, and invasion of HCC cells, as well as its underlying mechanism.
The expression levels of lncRNA TEX41 in HCC tissues are higher than those in adjacent tissues. The expression of lncRNA TEX41 was associated with lymph node metastasis and tumor-node-metastasis (TNM) staging in HCC patients. Silencing lncRNA TEX41 inhibited the proliferation, migration, and invasion of HCC cell lines. Low expression of lncRNA TEX41 may have affected epithelial-mesenchymal transition (EMT) in HCC cell lines. There may be a functional relationship between lncRNA TEX41 and miR-200a-3p-BIRC5.
LncRNA TEX41 may promote the progression of HCC through the miR-200a-3p-BIRC5 axis.
长链非编码RNA（lncRNA）TEX41与多种癌症的进展相关。在本研究中，我们在肝细胞癌（HCC）背景下检测了lncRNA TEX41的表达及其细胞生物学功能，以评估它们预测HCC进展的能力。进一步探讨了lncRNA TEX41、miR-200a-3p与潜在下游靶基因之间的关系。本研究的目的是为HCC的诊断和治疗提供新的理论依据。
利用GEPIA2、starBase和基因表达综合数据库（GEO）分析lncRNA TEX41的生物学功能和分子机制。通过定量实时聚合酶链反应（qRT-PCR）检测临床组织样本和细胞系中lncRNA TEX41的表达。我们研究了TEX41对HCC细胞增殖、迁移和侵袭的影响及其潜在机制。
HCC组织中lncRNA TEX41的表达水平高于相邻组织。lncRNA TEX41的表达与HCC患者的淋巴结转移和肿瘤-淋巴结-转移（TNM）分期相关。沉默lncRNA TEX41可抑制HCC细胞系的增殖、迁移和侵袭。lncRNA TEX41低表达可能影响了HCC细胞系中的上皮-间质转化（EMT）。lncRNA TEX41与miR-200a-3p-BIRC5之间可能存在功能关系。
LncRNA TEX41可能通过miR-200a-3p-BIRC5轴促进HCC的进展。
