Increased expression of predicts the poor prognosis of colorectal cancer patients.

BACKGROUND

Colorectal cancer (CRC) is considered one of the main causes of cancer-related deaths. Rho family-interacting cell polarization regulator 1 () is a new Rho effector protein, and its abnormal expression may be related to various tumors. However, its expression and significance in CRC are still unclear. We aimed to explore its role and potential value in CRC, and to provide a new theoretical basis of prognostic evaluation and targeted therapy for CRC patients.

METHODS

Here we investigated the expression and significance of from public databases by using bioinformatics methods. Immunohistochemistry (IHC) was performed to determine protein expression levels of in CRC patients, and the correlation between expression and clinicopathological characteristics was analyzed. cellular experiments were used to explore the role of in CRC cell lines.

RESULTS

Both bioinformatics analysis and IHC results demonstrated that was significantly overexpressed in CRC, which was associated with a worse prognosis. The microRNA (miRNA) database showed that hsa-miR-625-5p was significantly and negatively correlated with . may affect immune cell infiltration in CRC. Cell Counting Kit-8 (CCK-8) assay revealed that promoted cell proliferation in CRC.

CONCLUSIONS

has a significant impact on the development and proliferation of CRC, and may be a potential predictive biomarker.