Yue Xiaolei, Bian Junyu, Dong Zhikun, Chen Yonglin
Department of Pathology, The First Hospital of Lanzhou University, Lanzhou, China.
The First Clinical Medical College of Lanzhou University, Lanzhou, China.
Transl Cancer Res. 2025 May 30;14(5):2707-2721. doi: 10.21037/tcr-24-2029. Epub 2025 May 12.
Colorectal cancer (CRC) is considered one of the main causes of cancer-related deaths. Rho family-interacting cell polarization regulator 1 () is a new Rho effector protein, and its abnormal expression may be related to various tumors. However, its expression and significance in CRC are still unclear. We aimed to explore its role and potential value in CRC, and to provide a new theoretical basis of prognostic evaluation and targeted therapy for CRC patients.
Here we investigated the expression and significance of from public databases by using bioinformatics methods. Immunohistochemistry (IHC) was performed to determine protein expression levels of in CRC patients, and the correlation between expression and clinicopathological characteristics was analyzed. cellular experiments were used to explore the role of in CRC cell lines.
Both bioinformatics analysis and IHC results demonstrated that was significantly overexpressed in CRC, which was associated with a worse prognosis. The microRNA (miRNA) database showed that hsa-miR-625-5p was significantly and negatively correlated with . may affect immune cell infiltration in CRC. Cell Counting Kit-8 (CCK-8) assay revealed that promoted cell proliferation in CRC.
has a significant impact on the development and proliferation of CRC, and may be a potential predictive biomarker.
结直肠癌（CRC）被认为是癌症相关死亡的主要原因之一。Rho家族相互作用细胞极化调节因子1（）是一种新的Rho效应蛋白，其异常表达可能与多种肿瘤有关。然而，其在结直肠癌中的表达及意义仍不清楚。我们旨在探讨其在结直肠癌中的作用和潜在价值，为结直肠癌患者的预后评估和靶向治疗提供新的理论依据。
在此，我们使用生物信息学方法从公共数据库中研究的表达及意义。采用免疫组织化学（IHC）检测结直肠癌患者中蛋白表达水平，并分析表达与临床病理特征之间的相关性。利用细胞实验探讨在结直肠癌细胞系中的作用。
生物信息学分析和免疫组化结果均表明，在结直肠癌中显著高表达，这与较差的预后相关。微小RNA（miRNA）数据库显示，hsa-miR-625-5p与显著负相关。可能影响结直肠癌中的免疫细胞浸润。细胞计数试剂盒-8（CCK-8）检测显示，促进结直肠癌细胞增殖。
对结直肠癌的发生和增殖有显著影响，可能是一种潜在的预测生物标志物。
