Zhao Qian, Lu Yueting, Lu Hualin, Wang Dixian, Shang Hongyue, Yao Manman, Liu Tiejun
Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
Transl Cancer Res. 2025 May 30;14(5):2722-2736. doi: 10.21037/tcr-24-2025. Epub 2025 May 27.
Oral cancer ranks as the sixth most common malignancy worldwide, with a significantly higher prevalence among men aged 40 to 60 years than women. Previous studies have demonstrated that periplocin exhibits therapeutic potential by inhibiting cancer cell proliferation and inducing apoptosis across various cancer types. However, its role in oral squamous cell carcinoma (OSCC) and the associated molecular mechanisms remain insufficiently understood. In this study, we aimed to investigate the inhibitory effects of periplocin on the malignant behaviors of OSCC cells.
MTS [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide] assay, colony formation assay, flow cytometry, and transwell assay were employed to assess the effects of periplocin on OSCC cell proliferation, apoptosis, cell cycle progression, and migration. Furthermore, proteomics analysis was conducted to identify differentially expressed proteins in OSCC cells treated with periplocin, providing insights into its mechanisms of action.
Periplocin significantly influenced OSCC cell proliferation, apoptosis, cell cycle dynamics, and migration. Proteomics data indicated that periplocin modulates apoptosis, protein binding, and DNA replication signaling pathways. Furthermore, bioinformatics analysis revealed a strong association between syndecan 1 () and coiled-coil-helix-coiled-coil-helix domain containing 2 () in OSCC.
The findings of this study indicate that periplocin exerts anti-OSCC effects by modulating key cellular processes in OSCC cells, offering a promising therapeutic avenue for OSCC management.
口腔癌是全球第六大常见恶性肿瘤，40至60岁男性的患病率明显高于女性。先前的研究表明，杠柳毒苷通过抑制各种癌症类型的癌细胞增殖和诱导凋亡而具有治疗潜力。然而，其在口腔鳞状细胞癌（OSCC）中的作用及相关分子机制仍了解不足。在本研究中，我们旨在研究杠柳毒苷对OSCC细胞恶性行为的抑制作用。
采用MTS[3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四氮唑溴盐]法、集落形成试验、流式细胞术和Transwell试验来评估杠柳毒苷对OSCC细胞增殖、凋亡、细胞周期进程和迁移的影响。此外，进行蛋白质组学分析以鉴定经杠柳毒苷处理的OSCC细胞中差异表达的蛋白质，从而深入了解其作用机制。
杠柳毒苷显著影响OSCC细胞的增殖、凋亡、细胞周期动态和迁移。蛋白质组学数据表明，杠柳毒苷可调节凋亡、蛋白质结合和DNA复制信号通路。此外，生物信息学分析揭示了OSCC中syndecan 1（）与含卷曲螺旋-螺旋-卷曲螺旋结构域2（）之间存在强关联。
本研究结果表明，杠柳毒苷通过调节OSCC细胞中的关键细胞过程发挥抗OSCC作用，为OSCC的治疗提供了一条有前景的途径。
