Overexpression of promotes colorectal cancer growth.

BACKGROUND

Research has revealed that long non-coding RNAs (lncRNAs) are intimately associated with the occurrence, development, and metastasis of tumors through their regulation of gene expression. The lncRNA is important for colorectal cancer (CRC) occurrence and development. Our research aimed to explore the roles of in CRC progression.

METHODS

The expression of in CRC cells and tissues was first measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the prognosis of CRC patients was then investigated using the Kaplan-Meier method. The impacts of on CRC growth were evaluated by a series of and assays. Mechanistically, RNA sequencing (RNA-seq) technology and transcriptome analysis experiments were employed to validate the impact of on cell cycle pathway.

RESULTS

In this study, we have demonstrated, for the first time, that is significantly overexpressed in CRC and is associated with poor patient survival. Functional assays indicated that promotes the growth of CRC cells both and . Furthermore, RNA-seq has revealed the impact of on the cell cycle and DNA replication pathways, and identified MCM3 as a potential downstream target of .

CONCLUSIONS

Our findings indicate that is upregulated in CRC and promotes tumor growth.