Makhumbila Penny, Rauwane Molemi, Muedi Hangwani, Figlan Sandiswa
Department of Agriculture and Animal Health, School of Agriculture and Life Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Roodeport, South Africa.
Department of Botany, Nelson Mandela University, Port Elizabeth, South Africa.
Front Plant Sci. 2025 Jun 2;16:1557954. doi: 10.3389/fpls.2025.1557954. eCollection 2025.
Rust, caused by the obligate biotrophic fungal pathogen (Pers.:Pers.) Unger, is one of the most destructive diseases of common bean ( L.) causing yield losses in production areas worldwide. This study explores the use of RNA sequencing (RNA-seq) as a breeding prediction tool through the assessment of gene expression patterns of common bean susceptible (Golden Gate Wax) and resistant (Teebus-RR-1) varieties to virulent strain race 31-1 at two time points (14- and 21- days post infection; dpi). induced stress-responsive genes such as heat shock proteins (HSPs: and ), receptor-like kinases (RLKs: ), cytochrome monooxygenases (, and ) and terpene synthases ( and ) at 14-dpi, while and transcription factors, among others were regulated differentially in both varieties at 21-dpi. Genes , and were among the identified potential biomarkers associated with resistance. Enrichment of signaling receptor activity, response to stress, sesquiterpenoid and triterpenoid biosynthesis, and plant hormone signal transduction were unique to Teebus-RR-1. Overall, the findings of this study indicate varying gene expression patterns between varieties in response to . Insights provided by the RNA-Seq approach used in this study on mechanisms of response to can be used for biomarker discovery and possible development of resistant varieties.
由专性活体营养型真菌病原体（Pers.:Pers.）昂格尔引起的锈病，是菜豆（L.）最具毁灭性的病害之一，在全球各产区均会导致产量损失。本研究通过评估菜豆易感品种（金门蜡豆）和抗病品种（蒂布斯-RR-1）在两个时间点（感染后14天和21天；dpi）对强毒株31-1的基因表达模式，探索将RNA测序（RNA-seq）用作育种预测工具。在感染后14天诱导了热休克蛋白（HSPs: 和 ）、类受体激酶（RLKs: ）、细胞色素单加氧酶（, 和 ）和萜烯合酶（和 ）等应激反应基因，而在感染后21天，两个品种中 和 转录因子等的调控存在差异。基因, 和 是已鉴定出的与 抗性相关的潜在生物标志物。信号受体活性、对胁迫的反应、倍半萜和三萜生物合成以及植物激素信号转导的富集在蒂布斯-RR-1中是独特的。总体而言，本研究结果表明不同品种在响应 时基因表达模式存在差异。本研究中使用的RNA-Seq方法提供的关于 响应机制的见解可用于生物标志物发现以及抗性品种的可能培育。
