Insights of ' response to infection by using an RNA-seq approach.

Rust, caused by the obligate biotrophic fungal pathogen (Pers.:Pers.) Unger, is one of the most destructive diseases of common bean ( L.) causing yield losses in production areas worldwide. This study explores the use of RNA sequencing (RNA-seq) as a breeding prediction tool through the assessment of gene expression patterns of common bean susceptible (Golden Gate Wax) and resistant (Teebus-RR-1) varieties to virulent strain race 31-1 at two time points (14- and 21- days post infection; dpi). induced stress-responsive genes such as heat shock proteins (HSPs: and ), receptor-like kinases (RLKs: ), cytochrome monooxygenases (, and ) and terpene synthases ( and ) at 14-dpi, while and transcription factors, among others were regulated differentially in both varieties at 21-dpi. Genes , and were among the identified potential biomarkers associated with resistance. Enrichment of signaling receptor activity, response to stress, sesquiterpenoid and triterpenoid biosynthesis, and plant hormone signal transduction were unique to Teebus-RR-1. Overall, the findings of this study indicate varying gene expression patterns between varieties in response to . Insights provided by the RNA-Seq approach used in this study on mechanisms of response to can be used for biomarker discovery and possible development of resistant varieties.