A protocol for mapping epidemiology and diagnostics from One Health perspective.

is a globally prevalent gut protist colonising over a billion people worldwide, yet its epidemiology, transmission dynamics, and clinical significance remain underexplored. This protocol represents the first step of a large-scale effort to map epidemiology and diagnostic practices across Europe through the COST Action CA21105: . By assessing diagnostic methodologies across clinical, veterinary, and environmental sectors, this work sets the foundation for future research and standardisation. Here, we highlight key findings, challenges, and a roadmap for improving detection, ultimately influencing global health policies and microbial ecology studies.