Huang Wenbin, Toth Istvan, Skwarczynski Mariusz
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia.
Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia.
Methods Mol Biol. 2025;2931:343-354. doi: 10.1007/978-1-0716-4562-8_26.
Poly(hydrophobic amino acids) have been utilized as a component of peptide-based vaccines due to their ability to act as an immunostimulating moiety. They are biodegradable, possess defined stereochemistry and number of units, and upon conjugation to peptide antigens can self-assemble into nanoparticles. However, the process of synthesis and purification of the conjugates presents notable difficulties due to the highly hydrophobic nature of poly(hydrophobic amino acids), resulting in low production yield. Herein we present a protocol for the optimized automated synthesis and purification of these conjugates.
聚（疏水氨基酸）因其作为免疫刺激部分的能力，已被用作基于肽的疫苗的一个组成部分。它们是可生物降解的，具有明确的立体化学和单元数量，并且在与肽抗原缀合后可以自组装成纳米颗粒。然而，由于聚（疏水氨基酸）的高度疏水性，缀合物的合成和纯化过程存在显著困难，导致产量较低。在此，我们提出了一种优化的自动合成和纯化这些缀合物的方案。
