Ralston Corie Y, Gupta Sayan, Del Mundo Joshua T, Soe Aimee Chi, Russell Brandon, Rad Behzad, Tyler James, Paul Sathi, Kahan Darren N, Kristensen Line G, Subramanian Simruthi, Kidd Savannah, Burnett Kathryn, Sankaran Banumathi, Classen Scott, Prigozhin Daniil M, Taylor John R, Dickert Jeff M, Royal Kevin B, Rozales Anthony, Ortega Stacey L, Allaire Marc, Nix Jay C, Hura Greg L, Holton James M, Hammel Michal, Adams P D
Molecular Foundry Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
J Synchrotron Radiat. 2025 Jul 1;32(Pt 4):1059-1067. doi: 10.1107/S1600577525004205. Epub 2025 Jun 18.
ALS-ENABLE is an integrated NIH P30 resource at the Advanced Light Source synchrotron at Lawrence Berkeley National Laboratory in Berkeley, California, USA. The resource provides a single portal to the combined mature structural biology technologies of macromolecular crystallography, small-angle X-ray scattering and X-ray footprinting mass spectrometry, and includes beamlines 2.0.1, 3.3.1, 4.2.2, 5.0.1, 5.0.2, 5.0.3, 8.2.1, 8.2.2, 8.3.1 and 12.3.1. This paper describes the organizational structure and the technologies of ALS-ENABLE. A case study showcasing the main technologies of the resource applied to the characterization of the SpyCatcher-SpyTag protein system is presented.
ALS-ENABLE是美国加利福尼亚州伯克利市劳伦斯伯克利国家实验室先进光源同步加速器处的一个综合性美国国立卫生研究院P30资源。该资源为大分子晶体学、小角X射线散射和X射线足迹质谱等成熟结构生物学技术的组合提供了单一入口,包括2.0.1、3.3.1、4.2.2、5.0.1、5.0.2、5.0.3、8.2.1、8.2.2、8.3.1和12.3.1号光束线。本文描述了ALS-ENABLE的组织结构和技术。还展示了一个案例研究,该案例将该资源的主要技术应用于SpyCatcher-SpyTag蛋白质系统的表征。