Rahaman Mizanur, Strelkov Stephen E, Hu Hao, Fu Fuyou, Yu Fengqun
Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, Saskatoon, SK S7N 0X2, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Genome. 2025 Jan 1;68:1-12. doi: 10.1139/gen-2025-0017.
Clubroot, caused by the obligate parasite , poses a significant threat to the Canadian canola industry, resulting in substantial yield losses. Genetic resistance is an effective strategy for managing the disease. In this study, 137 doubled haploid (DH) lines derived from the European oilseed rape cultivar 'Mendel' were phenotyped against pathotypes 3D, 5C, and 8J, which are aggressive toward the first-generation clubroot-resistant canola cultivars, and were genotyped using genotyping-by-sequencing. Disease severity indices in the population were highly correlated among the three pathotypes, with correlation coefficients of ≥ 0.82. In the DH population, 2642 high-quality Single nucleotide polymorphisms were detected by employing the 'ZS11' reference genome. A single quantitative trait locus, , was detected and mapped to a 317 kb region on chromosome A08, flanked by the markers ZS_A08_15999175 and ZS_A08_16316110. Within this region, 43 genes were identified, including a single Toll interleukin-1 receptor nucleotide-binding site-leucine-rich repeat (TNL) gene, . This region is homologous to the 13 415 472-15 791 728 bp region of chromosome A08 in ECD 04, the donor of clubroot resistance in 'Mendel'. Two TNL genes, and , were identified in the ECD 04 genome. Kompetitive allele-specific PCR analysis identified eight markers that co-segregated with . Resistance to pathotype 3H was also found to co-segregate with resistance to pathotypes 5C, 3D, and 8J.
根肿病由专性寄生菌引起，对加拿大油菜产业构成重大威胁，导致产量大幅损失。基因抗性是防治该病的有效策略。在本研究中，对源自欧洲油菜品种“孟德尔”的137个双单倍体（DH）株系针对致病型3D、5C和8J进行了表型分析，这些致病型对第一代抗根肿病油菜品种具有侵袭性，并使用简化基因组测序进行了基因分型。群体中的病情严重程度指数在三种致病型之间高度相关，相关系数≥0.82。在DH群体中，利用“中双11号”参考基因组检测到2642个高质量单核苷酸多态性。检测到一个单数量性状位点，并将其定位到A08染色体上一个317 kb的区域，两侧分别是标记ZS_A08_15999175和ZS_A08_16316110。在该区域内，鉴定出43个基因，包括一个单Toll样白细胞介素-1受体核苷酸结合位点富含亮氨酸重复序列（TNL）基因。该区域与“孟德尔”根肿病抗性供体ECD 04中A08染色体的13415472 - 15791728 bp区域同源。在ECD 04基因组中鉴定出两个TNL基因和。竞争性等位基因特异性PCR分析鉴定出八个与共分离的标记。还发现对致病型3H的抗性与对致病型5C、3D和8J的抗性共分离。
