Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, 107 Science Place, Saskatoon, SK S7N OX2, Canada.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Int J Mol Sci. 2020 Jul 16;21(14):5033. doi: 10.3390/ijms21145033.
Genetic resistance is widely used to manage clubroot () in brassica crops, but new pathotypes have recently been identified on canola () on the Canadian prairies. Resistance effective against both the most prevalent pathotype (3H, based on the Canadian Clubroot Differential system) and the new pathotypes is needed. BC plants of from a cross of line 96-6990-2 (clubroot resistance originating from turnip cultivar 'Waaslander') and a susceptible doubled-haploid line, ACDC, exhibited a 1:1 segregation for resistance against pathotypes 3H and 5X. A resistance gene designated as was mapped initially based on the percentage of polymorphic variants using bulked segregant RNA sequencing (BSR-Seq) and further mapped using Kompetitive Allele Specific PCR. DNA variants were identified by assembling short reads against a reference genome of . was mapped into chromosome A08. It was flanked by single nucleotide polymorphisms (SNP) markers (A90_A08_SNP_M12 and M16) between 10.00 and 10.23 Mb, in an interval of 231.6 Kb. There were 32 genes in the interval. Three genes (, , and ) were annotated with disease resistance mechanisms, which are potential candidates for . Another resistance gene, designated as for resistance to pathotype 5X was mapped, with the flanking markers (A90_A08_SNP_M28 and M79) between 10.85 and 11.17 Mb using the SNP sites identified through BSR-Seq for . There were 44 genes in the interval, three of which () were annotated as immune-system-process related genes, which are potential candidates for .
遗传抗性被广泛用于防治芸薹属作物的根肿病，但最近在加拿大草原的油菜上发现了新的致病型。需要一种对最流行的致病型（基于加拿大根肿病鉴别系统的 3H）和新致病型都有效的抗性。来自于杂交种 96-6990-2（来自芜菁品种 'Waaslander' 的根肿病抗性）和易感双单倍体系 ACDC 的 BC 植株对 3H 和 5X 致病型表现出 1:1 的抗性分离。一个被命名为 的抗性基因最初是根据使用 bulked segregant RNA sequencing (BSR-Seq) 的多态性变异百分比进行定位的，然后使用 Kompetitive Allele Specific PCR 进一步定位。通过组装针对 参考基因组的短读序列鉴定了 DNA 变体。 将 定位到染色体 A08 上。它由 SNP 标记 (A90_A08_SNP_M12 和 M16) 侧翼，位于 10.00 和 10.23 Mb 之间，间隔 231.6 Kb。在 区间内有 32 个基因。三个基因（、和）被注释为具有疾病抗性机制，它们是 的潜在候选基因。另一个被命名为 的抗性基因，用于抗性 5X 致病型，其侧翼标记（A90_A08_SNP_M28 和 M79）位于 10.85 和 11.17 Mb 之间，使用通过 BSR-Seq 鉴定的 SNP 位点进行定位。在 区间内有 44 个基因，其中三个（）被注释为免疫系统过程相关基因，它们是 的潜在候选基因。
