Zhang Yuhan, Yu Jingjie, Wu Chenhuan, Jiang Xiaodan, Han Lehao, Yang Jiale, Zhang Shuqun, Han Junzi, Tai Yunru, Sun Yihan, Wang Boyan, Liu Yekai, Yan Yujing, Chen Qiang
State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.
School of Queen Mary, University of London Engineering, Northwestern Polytechnical University, Xi'an 710072, China.
Int J Biol Macromol. 2025 Jul;318(Pt 4):145229. doi: 10.1016/j.ijbiomac.2025.145229. Epub 2025 Jun 16.
Repairing large craniofacial bone defects presents a significant clinical challenge, primarily due to the elevated risk of bacterial infection and impaired osteogenic activity at the defect site. Herein, PLA-Ag@Sr composite nanofiber membranes with dual osteogenic and antibacterial properties for treating infected skull defects are reported, achieved by integrating dual-function core-shell Ag@Sr mesoporous silica nanoparticles (Ag@MSN@Sr) into PLA nanofibers via one-pot electrospinning method, effectively addressing the limitations of single functionality, structural complexity, and poor stability inherent in conventional multi-component GBR membranes. PLA-Ag@Sr exhibits favorable mechanical properties, moderate hydrophilicity, and sustained release of Ag and Sr ions. In vitro cellular assays indicated that the PLA-Ag@Sr possesses excellent biocompatibility and enhances osteogenic differentiation. Antibacterial assessments further confirmed the effective antibacterial activity of PLA-Ag@Sr in both in vitro and in vivo. Moreover, evaluations on calvarial defect rat model demonstrated the outstanding biocompatibility and significant osteogenesis potential of PLA-Ag@Sr. The synergistic combination of Sr (for osteogenic stimulation) and Ag (for broad-spectrum antibacterial action) in our core-shell system provides a new "kill two birds with one stone" idea with balanced osteogenesis and antibacterial properties for treating infected bone defects.
修复大型颅面骨缺损是一项重大的临床挑战，主要是因为细菌感染风险升高以及缺损部位的成骨活性受损。本文报道了具有双重成骨和抗菌特性的PLA-Ag@Sr复合纳米纤维膜用于治疗感染性颅骨缺损，通过一锅法静电纺丝将双功能核壳Ag@Sr介孔二氧化硅纳米颗粒（Ag@MSN@Sr）整合到PLA纳米纤维中实现，有效解决了传统多组分引导骨再生（GBR）膜固有的单一功能、结构复杂和稳定性差的局限性。PLA-Ag@Sr具有良好的机械性能、适度的亲水性以及Ag和Sr离子的持续释放。体外细胞实验表明，PLA-Ag@Sr具有优异的生物相容性并能增强成骨分化。抗菌评估进一步证实了PLA-Ag@Sr在体外和体内均具有有效的抗菌活性。此外，对颅骨缺损大鼠模型的评估证明了PLA-Ag@Sr具有出色的生物相容性和显著的成骨潜力。我们核壳系统中Sr（用于成骨刺激）和Ag（用于广谱抗菌作用）的协同组合为治疗感染性骨缺损提供了一种新的“一石二鸟”理念，即具有平衡的成骨和抗菌特性。
