Zhou Peiqian, Liu Tuozhou, Liu Wenbin, Sun Lingshun, Kang Haifei, Liu Kun, Luo Peiyuan, Wang Youfa, Luo Ling, Dai Honglian
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China.
School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou 511442, China.
ACS Appl Mater Interfaces. 2024 Apr 16. doi: 10.1021/acsami.4c01206.
The periosteum, rich in neurovascular networks, bone progenitor cells, and stem cells, is vital for bone repair. Current artificial periosteal materials face challenges in mechanical strength, bacterial infection, and promoting osteogenic differentiation and angiogenesis. To address these issues, we adjusted the electrospinning ratio of poly-ε-caprolactone and chitosan and incorporated Zn doping whitlockite with polydopamine coating into a nanofiber membrane. After a series of characterizations, optimal results were achieved with a poly-ε-caprolactone: chitosan ratio of 8:1 and 5% nanoparticle content. In vitro cell experiments and in vivo calvarial defect models, the sustained release of Mg and Ca promoted vascularization and new bone formation, respectively, while the release of Zn was conducive to antibacterial and cooperated with Mg to promote neurovascularization. Consequently, this antibacterial bionic periosteum with an angiogenesis-neurogenesis coupling effect demonstrates a promising potential for bone repair applications.
骨膜富含神经血管网络、骨祖细胞和干细胞，对骨修复至关重要。目前的人工骨膜材料在机械强度、细菌感染以及促进成骨分化和血管生成方面面临挑战。为了解决这些问题，我们调整了聚ε-己内酯和壳聚糖的静电纺丝比例，并将掺锌白磷钙铁矿与聚多巴胺涂层结合到纳米纤维膜中。经过一系列表征，聚ε-己内酯与壳聚糖比例为8:1且纳米颗粒含量为5%时取得了最佳结果。在体外细胞实验和体内颅骨缺损模型中，镁和钙的持续释放分别促进了血管生成和新骨形成，而锌的释放有利于抗菌并与镁协同促进神经血管生成。因此，这种具有血管生成-神经生成耦合效应的抗菌仿生骨膜在骨修复应用中显示出有前景的潜力。
