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从西班牙加那利群岛特内里费岛的环境样本中分离和分子鉴定致病性自由生活阿米巴

Isolation and Molecular Identification of Pathogenic Free-Living Amoebae from Environmental Samples in Tenerife, Canary Islands, Spain.

作者信息

Pérez-Pérez Patricia, Reyes-Batlle María, Morchón Rodrigo, Piñero José E, Lorenzo-Morales Jacob

机构信息

Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias (IUETSPC), Universidad de La Laguna (ULL), Avenida Astrofísico Francisco Sánchez S/N, San Cristóbal de La Laguna, 38206 Tenerife, Spain.

Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Facultad de Farmacia, Universidad de La Laguna, San Cristóbal de La Laguna 38200 Tenerife, Spain.

出版信息

ACS ES T Water. 2025 May 2;5(6):2861-2869. doi: 10.1021/acsestwater.4c00573. eCollection 2025 Jun 13.

DOI:10.1021/acsestwater.4c00573
PMID:40535166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12172190/
Abstract

Free-living amoebae (FLA) are protozoa that have been reported worldwide from various environmental sources. However, only some groups (Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris, Sappinia pedata, Vahlkampfia spp., and Vermamoeba vermiformis) are considered to be pathogenic for humans and other animals. FLA has been previously reported in the Canary Islands archipelago since 2005. However, climate change and other environmental factors that could affect the communities of FLA in the islands lead to the development of this extra study. This study was carried out in Tenerife, the largest and most populated island, between June 2021 and July 2022. A total of 28 soil samples and 23 water samples were seeded in 2% non-nutrient agar (NNA) plates, incubated at 26 °C, and monitored daily to evaluate the presence of FLA. DNA was extracted from those plates on which there was suspected FLA growth, and polymerase chain reaction amplification of the 18S rRNA gene (DF3• region on the case of Acanthamoeba) was carried out. The obtained results showed that the genus Acanthamoeba (25/51:48.07%), with the genotype T4 being the most common (21/25; 84%), and the species V. vermiformis (7/51; 13.73%) were the most abundant FLA in the surveyed samples collected from Tenerife.

摘要

自由生活阿米巴（FLA）是原生动物，已在世界各地的各种环境来源中被报道。然而，只有一些类群（棘阿米巴属、福氏耐格里阿米巴、曼氏巴兰姆希阿米巴、佩氏萨平虫、瓦氏变形虫属和蠕虫样变形虫）被认为对人类和其他动物具有致病性。自2005年以来，加那利群岛群岛此前已报道过FLA。然而，气候变化和其他可能影响该群岛FLA群落的环境因素促使开展了这项额外研究。这项研究于2021年6月至2022年7月在特内里费岛（最大且人口最多的岛屿）进行。总共28份土壤样本和23份水样接种于2%非营养琼脂（NNA）平板中，在26℃下培养，并每日监测以评估FLA的存在情况。从疑似有FLA生长的平板中提取DNA，并对18S rRNA基因（棘阿米巴属情况下的DF3•区域）进行聚合酶链反应扩增。所得结果表明，在从特内里费岛采集的被调查样本中，棘阿米巴属（25/51:48.07%）是最丰富的FLA，其中基因型T4最为常见（21/25；84%），蠕虫样变形虫（7/51；13.73%）次之。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/43de77731fdb/ew4c00573_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/ed9cd45d3131/ew4c00573_0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/12677fa07c8b/ew4c00573_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/7f1dd24d7203/ew4c00573_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/43de77731fdb/ew4c00573_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/ed9cd45d3131/ew4c00573_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/30708b3eb3f2/ew4c00573_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/add843b006dd/ew4c00573_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/c95e14d60301/ew4c00573_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/0a6ab6215fe3/ew4c00573_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/12677fa07c8b/ew4c00573_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/7f1dd24d7203/ew4c00573_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081c/12172190/43de77731fdb/ew4c00573_0008.jpg

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