虹鳟（鲑鱼）肾脏中的谷胱甘肽S-转移酶活性。

The glutathione S-transferase activity in the kidney of rainbow trout (Salmo gairdneri).

作者信息

Nimmo I A, Spalding C M

出版信息

Comp Biochem Physiol B. 1985;82(1):91-4. doi: 10.1016/0305-0491(85)90133-6.

Abstract

Trout kidney contains 2.3 mmol GSH/kg. The cytosolic glutathione S-transferase activity with 1-chloro-2,4-dinitrobenzene as substrate is 0.35 mumol/min/mg protein. There is no detectable activity with 1,2-epoxy-3-(p-nitrophenoxy)propane, ethacrynic acid, p-nitrobenzyl chloride or p-nitrophenyl acetate. A variable proportion of the activity does not bind to a glutathione-affinity matrix. Its Km values for GSH and 1-chloro-2,4-dinitrobenzene are 3.0 and 5.1 mM, respectively. The rest of the activity is eluted from the affinity matrix as one main and two minor peaks. The main peak has Km values for GSH and 1-chloro-2,4-dinitrobenzene of 0.4 and 4.5 mM, respectively. Its subunit Mr is 22,900. The activity in the main peak is inhibited progressively by 1-chloro-2,4-dinitrobenzene with a rate constant of 0.11/min.

摘要

虹鳟鱼肾脏中谷胱甘肽（GSH）含量为2.3 mmol/kg。以1-氯-2,4-二硝基苯为底物时，胞质谷胱甘肽S-转移酶的活性为0.35 μmol/（min·mg蛋白）。对于1,2-环氧-3-（对硝基苯氧基）丙烷、依他尼酸、对硝基苄基氯或对硝基苯乙酸，未检测到活性。有一部分活性不与谷胱甘肽亲和基质结合。其对GSH和1-氯-2,4-二硝基苯的米氏常数（Km）分别为3.0 mM和5.1 mM。其余活性从亲和基质上洗脱下来，形成一个主峰和两个小峰。主峰对GSH和1-氯-2,4-二硝基苯的Km值分别为0.4 mM和4.5 mM。其亚基的相对分子质量（Mr）为22,900。主峰中的活性被1-氯-2,4-二硝基苯逐渐抑制，抑制速率常数为0.11/min。