Ullah Mati, Naeem Muhammad, Andoh Vivian, Khan Muhammad Nadeem, Han Jianda, Rizwan Muhammad, Hussain Nazar, Saeed Muhammad, Chen Yong, Chen Huayou
School of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212000, P. R. China.
Department of Cell Biology and Genetics, Shantou University Medical College, Shantou 515041, P.R. China.
J Agric Food Chem. 2025 Jul 2;73(26):16028-16048. doi: 10.1021/acs.jafc.5c02311. Epub 2025 Jun 19.
Lactic acid bacteria (LAB) constitute a genetically heterogeneous group that is uniquely capable of converting soluble carbohydrates into lactic acid. Such LAB, with a long history of safe consumption in fermented foods, are considered food-grade microorganisms and are highly sought after for a variety of biotechnological applications. Due to their unique properties, LAB can be genetically engineered to produce industrially significant enzymes. LAB act as an expression host for these enzymes by combining already existing engineering systems with techniques such as CRISPR-Cas. This review outlines the progress achieved to date on genome manipulation methods for LAB engineering and future perspectives of genetic tools. These strategies contribute greatly to fully unleashing the potential of LAB, and we further elaborate on how genome editing tools can enhance the capacity of heterologous expression in LAB.
乳酸菌（LAB）是一组基因异质性的菌群，具有将可溶性碳水化合物转化为乳酸的独特能力。这类乳酸菌在发酵食品中有着长期安全食用的历史，被视为食品级微生物，在各种生物技术应用中备受青睐。由于其独特的特性，乳酸菌可通过基因工程来生产具有工业意义的酶。通过将现有的工程系统与CRISPR-Cas等技术相结合，乳酸菌可作为这些酶的表达宿主。本综述概述了迄今为止在乳酸菌工程基因组操作方法方面取得的进展以及遗传工具的未来前景。这些策略对充分释放乳酸菌的潜力有很大贡献，我们还将进一步阐述基因组编辑工具如何提高乳酸菌中异源表达的能力。
