Talukdar Pinku Mani, Sonowal Dharitree, Sharma Ajanta, Upadhyaya Deepak, Kumar Sachin, Kaur Harpreet
Multidisciplinary Research Unit, Gauhati Medical College, Guwahati, Assam, India.
Department of Microbiology, Gauhati Medical College, Guwahati, Assam, India.
Indian J Med Res. 2025 Apr;161(4):386-393. doi: 10.25259/IJMR_1410_2024.
Background & objectives We aimed to conduct a comprehensive analysis of the cytokine profile in Japanese encephalitis (JE) patients and healthy individuals. Additionally, the correlation between the cytokines and the disease outcome in terms of survival or non-survival was also studied. Methods The study included 72 laboratory-confirmed JE cases and 50 healthy controls. Plasma levels of cytokines viz., GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, and TNF-α were analysed using Bio-plex200 (Bio-Rad) following manufacturer's guidelines and compared between JE patients and healthy control group. Additionally, quantitative real-time PCR (qRT-PCR) was done for the quantification of expression of the above-mentioned cytokine genes. Results Except IL-4 and IL-13, the levels of GM-CSF, IFN-γ, IL-2, IL-5, IL-10, IL-12, and TNF-α were significantly higher in JE patients in comparison to healthy controls. Significantly upregulated expression of IL-12, IL-10, and TNF-α was observed in the JE group as compared to that in healthy controls. Additionally, significantly downregulated expression of IL-4and IL-13 was observed in the JE group compared to the control group. Interpretation & conclusions A higher level of several pro-inflammatory cytokines and downregulation of a few anti-inflammatory cytokines were observed in JE patients compared to the healthy controls indicating co-association of inflammation with disease severity. Hence, a regulator of these pro and anti-inflammatory cytokines may stand out as a potential candidate for therapy in JE.
我们旨在对日本脑炎（JE）患者和健康个体的细胞因子谱进行全面分析。此外，还研究了细胞因子与生存或非生存疾病结局之间的相关性。方法：该研究纳入了72例实验室确诊的JE病例和50例健康对照。按照制造商的指南，使用Bio-plex200（伯乐公司）分析血浆中GM-CSF、IFN-γ、IL-2、IL-4、IL-5、IL-10、IL-12、IL-13和TNF-α等细胞因子的水平，并在JE患者和健康对照组之间进行比较。此外，进行定量实时PCR（qRT-PCR）以定量上述细胞因子基因的表达。结果：与健康对照相比，除IL-4和IL-13外，JE患者中GM-CSF、IFN-γ、IL-2、IL-5、IL-10、IL-12和TNF-α的水平显著更高。与健康对照相比，JE组中IL-12、IL-10和TNF-α的表达显著上调。此外，与对照组相比，JE组中IL-4和IL-13的表达显著下调。解读与结论：与健康对照相比，JE患者中观察到几种促炎细胞因子水平较高，而一些抗炎细胞因子下调，这表明炎症与疾病严重程度共同关联。因此，这些促炎和抗炎细胞因子的调节剂可能成为JE治疗的潜在候选药物。
