Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan.
Tropical Medicine Center, Kaohsiung Medical University Hospital, Kaohsiung 807378, Taiwan.
Int J Mol Sci. 2021 Mar 12;22(6):2879. doi: 10.3390/ijms22062879.
Clinical presentations of dengue fever (DF) are diverse and non-specific, causing unpredictable progression and outcomes. Its progression and severity have been associated with cytokine levels alteration. In this study, dengue patients were classified into groups following the 2009 WHO dengue classification scheme to investigate the cytokine signature at different severity of the disease: dengue without warning sign symptoms (A); dengue with warning signs (B); severe dengue (C); other fever (OF) and healthy (Healthy). We analyzed 23 different cytokines simultaneously, namely IL-1b, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-33, CD14, CD54, CD62E, CD62L, CD62p, CD106, CD121b, CD154, CD178, GM-CSF, IFN-g, MIF, ST2 and TNF from patients admitted to National Cheng Kung University Hospital during the 2015 Taiwan dengue outbreak. Cytokines TNF, CD54, CD62E, CD62L, CD62P, GM-CSF, IL-1b, IL-2, IL-6, IL-8, IL-10, IL-12p70, IL-17A, INF-g and MIF were elevated while CD106, CD154, IL-4 and L-33 were decreased when compared to the control. IL-10 demonstrated to be a potential diagnostic marker for DF (H and A group; AUC = 0.944, H and OF group; AUC = 0.969). CD121b demonstrated to be predictive of the SD (A and B group; AUC = 0.744, B and C group; AUC = 0.775). Our results demonstrate the cytokine profile changes during the progression of dengue and highlight possible biomarkers for optimizing effective intervention strategies.
登革热（DF）的临床症状多种多样且不具特异性，导致其病程和结果难以预测。其病程和严重程度与细胞因子水平的改变有关。在这项研究中，根据 2009 年世卫组织登革热分类方案，登革热患者被分为不同组别，以研究不同严重程度疾病的细胞因子特征：无症状登革热（A）；有警告症状的登革热（B）；重症登革热（C）；其他发热（OF）和健康对照（Healthy）。我们同时分析了 23 种不同的细胞因子，即白细胞介素-1b（IL-1b）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-10（IL-10）、白细胞介素-12p70（IL-12p70）、白细胞介素-17A（IL-17A）、白细胞介素-33（IL-33）、CD14、CD54、CD62E、CD62L、CD62P、CD106、CD121b、CD154、CD178、GM-CSF、IFN-g、MIF、ST2 和 TNF，这些患者均于 2015 年台湾登革热疫情期间入住国立成功大学医院。与对照组相比，TNF、CD54、CD62E、CD62L、CD62P、GM-CSF、IL-1b、IL-2、IL-6、IL-8、IL-10、IL-12p70、IL-17A、INF-g 和 MIF 等细胞因子升高，而 CD106、CD154、IL-4 和 IL-33 降低。IL-10 可作为 DF（H 和 A 组；AUC=0.944，H 和 OF 组；AUC=0.969）的潜在诊断标志物。CD121b 可预测 SD（A 和 B 组；AUC=0.744，B 和 C 组；AUC=0.775）。我们的研究结果表明登革热病程中细胞因子谱的变化，并强调了可能的生物标志物，以优化有效的干预策略。
