Chen Chaoxi, A-Ga Yue Bu Mo, Kuang Xiuhua, Li Ye, Xiang Rong, Liang Jun
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, China.
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
Vet Med Sci. 2025 Jul;11(4):e70416. doi: 10.1002/vms3.70416.
Palmatine is an isoquinoline alkaloid isolated from many medicinal plants with diverse pharmacological activities, with potential for the treatment of chronic metabolic disorders and infectious diseases. In the present study, a highly rapid, simple, and sensitive ultra-performance liquid chromatography coupled with an ultra-violet (UPLC-UV) method was developed and validated for the pharmacokinetics and tissue distribution of palmatine following single intramuscular injection to Partridge Shank chickens. The process was sensitive, with a lower limit of quantification and good linearity over the range of 0.005-10 µg/mL for UPLC-UV in all biological matrices. Following a single intramuscular dose of 5 mg/kg b.w, palmatine was absorbed quickly and reached peak concentration (0.24 µg/mL) at 0.19 h and was still present in plasma at 12 h with a concentration of 0.011 µg/mL. The CL/F and Vd/F were 10.43 L·h·kg and 56.69 L/kg, respectively. The C values of palmatine in tissues decreased as follows: kidneys > liver > spleen > heart > lung > muscles, and were almost completely cleared from lungs 26 h after administration and were not detectable or significantly reduced in other tissues after 48 h. The results can provide a basis for exploring the therapeutic potential of palmatine and looking for new ways of wide clinical applications in poultry farming.
巴马汀是一种从多种药用植物中分离出来的异喹啉生物碱，具有多种药理活性，具有治疗慢性代谢紊乱和传染病的潜力。在本研究中，建立了一种高效、快速、简单且灵敏的超高效液相色谱-紫外联用（UPLC-UV）方法，并对其进行了验证，用于测定鹧鸪胫鸡单次肌肉注射巴马汀后的药代动力学和组织分布。该方法灵敏，在所有生物基质中，UPLC-UV的定量下限较低，在0.005-10 µg/mL范围内具有良好的线性。单次肌肉注射剂量为5 mg/kg体重后，巴马汀吸收迅速，在0.19 h达到峰值浓度（0.24 µg/mL），12 h时血浆中仍有浓度为0.011 µg/mL的巴马汀。CL/F和Vd/F分别为10.43 L·h·kg 和56.69 L/kg。巴马汀在组织中的C值下降顺序如下：肾脏>肝脏>脾脏>心脏>肺>肌肉，给药后26 h肺部几乎完全清除，48 h后其他组织中未检测到或显著降低。这些结果可为探索巴马汀的治疗潜力以及寻找家禽养殖中广泛临床应用的新途径提供依据。
