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金黄色葡萄球菌H和枯草芽孢杆菌W23中核糖醇磷壁酸生物合成中连接单元中间体的结构与功能

Structure and functions of linkage unit intermediates in the biosynthesis of ribitol teichoic acids in Staphylococcus aureus H and Bacillus subtilis W23.

作者信息

Yokoyama K, Miyashita T, Araki Y, Ito E

出版信息

Eur J Biochem. 1986 Dec 1;161(2):479-89. doi: 10.1111/j.1432-1033.1986.tb10469.x.

DOI:10.1111/j.1432-1033.1986.tb10469.x
PMID:3096735
Abstract

The stepwise formation and characterization of linkage unit intermediates and their functions in ribitol teichoic acid biosynthesis were studied with membranes obtained from Staphylococcus aureus H and Bacillus subtilis W23. The formation of labeled polymer from CDP-[14C]ribitol and CDP-glycerol in each membrane system was markedly stimulated by the addition of N-acetylmannosaminyl(beta 1----4)N-acetylglucosamine (ManNAc-GlcNAc) linked to pyrophosphorylyisoprenol. Whereas incubation of S. aureus membranes with CDP-glycerol and ManNAc-[14C]GlcNAc-PP-prenol led to synthesis of (glycerol phosphate) 1-3-ManNAc-[14C]GlcNAc-PP-prenol, incubation of B. subtilis membranes with the same substrates yielded (glycerol phosphate)1-2-ManNAc-[14C]GlcNAc-PP-prenol. In S. aureus membranes, (glycerol phosphate)2-ManNAc-[14C]GlcNAc-PP-prenol as well as (glycerol phosphate)3-ManNAc-[14C]GlcNAc-PP-prenol served as an acceptor for ribitol phosphate units, but (glycerol phosphate)-ManNAc-[14C]GlcNAc-PP-prenol did not. In B. subtilis W23 membranes, (glycerol phosphate)-ManNAc-[14C]GlcNAc-PP-prenol served as a better acceptor for ribitol phosphate units than (glycerol phosphate)2-ManNAc-[14C]GlcNAc-PP-prenol. In this membrane system (ribitol phosphate)-(glycerol phosphate)-ManNAc-[14C]GlcNAc-PP-prenol was formed from ManNAc-[14C]GlcNAc-PP-prenol, CDP-glycerol and CDP-ribitol. The results indicate that (glycerol phosphate)1-3-ManNAc-GlcNAc-PP-prenol and (glycerol phosphate)1-2-ManNac-GlcNAc-PP-prenol are involved in the pathway for the synthesis of wall ribitol teichoic acids in S. aureus H and B. subtilis W23 respectively.

摘要

利用从金黄色葡萄球菌H和枯草芽孢杆菌W23获得的细胞膜，研究了连接单元中间体的逐步形成、表征及其在核糖醇磷壁酸生物合成中的功能。在每个膜系统中，通过添加与焦磷酸异戊烯醇连接的N-乙酰甘露糖胺基（β1→4）N-乙酰葡糖胺（ManNAc-GlcNAc），显著刺激了由CDP-[14C]核糖醇和CDP-甘油形成标记聚合物。当用CDP-甘油和ManNAc-[14C]GlcNAc-PP-异戊烯醇孵育金黄色葡萄球菌细胞膜时，导致合成（甘油磷酸）1-3-ManNAc-[14C]GlcNAc-PP-异戊烯醇，而用相同底物孵育枯草芽孢杆菌细胞膜则产生（甘油磷酸）1-2-ManNAc-[14C]GlcNAc-PP-异戊烯醇。在金黄色葡萄球菌细胞膜中，（甘油磷酸）2-ManNAc-[14C]GlcNAc-PP-异戊烯醇以及（甘油磷酸）3-ManNAc-[14C]GlcNAc-PP-异戊烯醇可作为磷酸核糖醇单元的受体，但（甘油磷酸）-ManNAc-[14C]GlcNAc-PP-异戊烯醇则不能。在枯草芽孢杆菌W23细胞膜中，（甘油磷酸）-ManNAc-[14C]GlcNAc-PP-异戊烯醇作为磷酸核糖醇单元的受体比（甘油磷酸）2-ManNAc-[14C]GlcNAc-PP-异戊烯醇更好。在该膜系统中，由ManNAc-[14C]GlcNAc-PP-异戊烯醇、CDP-甘油和CDP-核糖醇形成了（磷酸核糖醇）-（甘油磷酸）-ManNAc-[14C]GlcNAc-PP-异戊烯醇。结果表明，（甘油磷酸）1-3-ManNAc-GlcNAc-PP-异戊烯醇和（甘油磷酸）1-2-ManNac-GlcNAc-PP-异戊烯醇分别参与了金黄色葡萄球菌H和枯草芽孢杆菌W23中壁核糖醇磷壁酸的合成途径。

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