Hancock I, Baddiley J
J Bacteriol. 1976 Mar;125(3):880-6. doi: 10.1128/jb.125.3.880-886.1976.
The role of cytidine diphosphate (CDP)-glycerol in gram-positive bacteria whose walls lack poly(glycerol phosphate) was investigated. Membrane preparations from Staphylococcus aureus H, Bacillus subtilis W23, and Micrococcus sp. 2102 catalyzed the incorporation of glycerol phosphate residues from radioactive CDP-glycerol into a water-soluble polymer. In toluenized cells of Micrococcus sp. 2102, some of this product became linked to the wall. In each case, maximum incorporation of glycerol phosphate residues required the presence of the nucleotide precursors of wall teichoic acid and of uridine diphosphate-N-acetylglucosamine. In membrane preparations capable of synthesizing peptidoglycan, vancomycin caused a decrease in the incorporation of isotope from CDP-glycerol into polymer. Synthesis of the poly (glycerol phosphate) unit thus depended at an early stage on the concomitant synthesis of wall teichoic acid and later on the synthesis of peptidoglycan. It is concluded that CDP-glycerol is the biosynthetic precursor of the tri(glycerol phosphate) linkage unit between teichoic acid and peptidoglycan that has recently been characterized in S. aureus H.
研究了胞苷二磷酸（CDP）-甘油在细胞壁缺乏聚（甘油磷酸）的革兰氏阳性菌中的作用。从金黄色葡萄球菌H、枯草芽孢杆菌W23和微球菌属2102制备的膜制剂催化了放射性CDP-甘油中的甘油磷酸残基掺入一种水溶性聚合物中。在微球菌属2102的甲苯化细胞中，部分该产物与细胞壁相连。在每种情况下，甘油磷酸残基的最大掺入需要壁磷壁酸的核苷酸前体和尿苷二磷酸-N-乙酰葡糖胺的存在。在能够合成肽聚糖的膜制剂中，万古霉素导致从CDP-甘油掺入聚合物中的同位素减少。因此，聚（甘油磷酸）单元的合成在早期阶段依赖于壁磷壁酸的同时合成，后期依赖于肽聚糖的合成。得出的结论是，CDP-甘油是磷壁酸和肽聚糖之间最近在金黄色葡萄球菌H中鉴定出的三（甘油磷酸）连接单元的生物合成前体。
