单次高强度力量与耐力运动对年轻成年人和老年男性认知生物标志物的急性影响：一项受试者内交叉研究。

Acute effects of a single bout of high-intensity strength and endurance exercise on cognitive biomarkers in young adults and elderly men: a within-subjects crossover study.

作者信息

Bekkos Carolin Haberstroh, Sujan Md Abu Jafar, Stunes Astrid Kamilla, Tari Atefe Rafiee, Aagård Norun, Brobakken Cathrine Langlie, Brevig Martin Siksjø, Syversen Unni, Wang Eivind, Mosti Mats Peder

机构信息

Department of Research and Development, Clinic of Substance Use and Addiction Medicine, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.

Department of Mental Health, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

出版信息

J Transl Med. 2025 Jun 19;23(1):685. doi: 10.1186/s12967-025-06685-y.

DOI:10.1186/s12967-025-06685-y

PMID:40537774

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12177964/

Abstract

BACKGROUND

Although evidence for exercise-induced changes in neurocognitive biomarkers is emerging, research examining acute responses to different exercise regimes across sex and age is lacking. This study investigated serum concentrations of three neurocognitive biomarkers (i.e., Klotho, brain-derived neurotrophic factor (BDNF), and glycosylphosphatidylinositol-specific phospholipase D1 (GPLD1)) after acute strength and aerobic exercise, along with skeletal muscle gene expression.

METHODS

In a within-subjects crossover design, blood samples of 19 young women, 20 young men, and 14 elderly men were taken before, immediately, 3 h and 24 h after one bout of strength training (ST) and high-intensity interval training (HIIT). Muscle biopsies were taken from a subgroup (n = 22) before, 3 h and 24 h after ST and HIIT for gene expression analyses. Time changes and baseline levels, including the influence of sex and age, were analyzed using a multilevel model and Welch's analysis of variance, respectively. Biomarker levels were adjusted for exercise-induced plasma volume changes.

RESULTS

Serum concentration of all biomarkers increased after ST and HIIT but were not affected by sex or age. While serum Klotho and BDNF levels peaked immediately after exercise in all groups, serum GPLD1 levels were highest at 3 h (young groups only). Age was a determining factor for baseline measures; young men had higher and lower resting serum Klotho and BDNF concentration, respectively, than elderly men. Muscle gene expression of Klotho increased after both exercise modes, and BDNF and GPLD1 expression was reduced within 24 h.

CONCLUSIONS

Circulating levels of biomarkers linked to brain health can acutely be increased by one bout of ST or HIIT. This increase might be related to altered gene expression of these proteins in skeletal muscle. Ultimately, this could have beneficial implications for the management of mental and neurocognitive impairments.

摘要

背景

尽管运动引起的神经认知生物标志物变化的证据正在出现，但缺乏针对不同运动方案在性别和年龄方面的急性反应的研究。本研究调查了急性力量训练和有氧运动后三种神经认知生物标志物（即 Klotho、脑源性神经营养因子 (BDNF) 和糖基磷脂酰肌醇特异性磷脂酶 D1 (GPLD1)）的血清浓度，以及骨骼肌基因表达。

方法

在一项受试者内交叉设计中，在 19 名年轻女性、20 名年轻男性和 14 名老年男性进行一轮力量训练 (ST) 和高强度间歇训练 (HIIT) 之前、之后立即、3 小时和 24 小时采集血样。在 ST 和 HIIT 之前、3 小时和 24 小时从一个亚组（n = 22）采集肌肉活检样本用于基因表达分析。分别使用多级模型和 Welch 方差分析来分析时间变化和基线水平，包括性别和年龄的影响。对生物标志物水平进行了运动引起的血浆容量变化的校正。

结果

ST 和 HIIT 后所有生物标志物的血清浓度均升高，但不受性别或年龄影响。虽然所有组的血清 Klotho 和 BDNF 水平在运动后立即达到峰值，但血清 GPLD1 水平在 3 小时时最高（仅年轻组）。年龄是基线测量的决定因素；年轻男性的静息血清 Klotho 和 BDNF 浓度分别高于和低于老年男性。两种运动模式后 Klotho 的肌肉基因表达均增加，BDNF 和 GPLD1 的表达在 24 小时内降低。