Jo Seung-Woo, Velankanni Priyanka, Cam Nhung Do Thi, Nguyen Chau Hoang Bao, Jeon Jin-Young, Kim Bo-Ra, Lee Dae-Hee, Lee Choong-Gu, Park Jin-Soo
Center for Natural Product Systems Biology, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of Korea.
Department of Marine Bio Food Science, Gangneung-Wonju National University, Gangneung 25457, Republic of Korea.
J Microbiol Biotechnol. 2025 Jun 12;35:e2503021. doi: 10.4014/jmb.2503.03021.
Chlorella, a genus of single-celled green algae, is widely industrially cultivated as a dietary supplement ingredient due to its high protein content and various nutrients. In this study, the immune-stimulating effect of solvent fractions prepared from (CV) and (CP) was evaluated through the expression of immune-related factors, such as TNF-α and IFN-γ in T cells obtained from mice. Both species showed immunostimulatory effects in hexane and butanol fractions, but CV increased TNF-α only, while CP elevated both TNF-α and IFN-γ. In addition, the lipid profile of was characterized using non-selective LC-MS/MS-based metabolomic analysis. According to the lipidomic results, the two species differed in lipid composition, with PC 32:1, PC 32:2, PC 34:5, DGDG 34:4, and DGDG 34:5 being enriched in the active fractions of CV, and PC 34:1, PC 34:2, PC O-15:2 and DGTS 34:4 being abundant in the fractions of CP. Furthermore, 5'-methylthioadenosine (MTA), which was detected in both species, also exhibited immunostimulatory properties.
小球藻是一种单细胞绿藻，由于其高蛋白含量和多种营养成分，在工业上被广泛用作膳食补充剂成分进行培养。在本研究中，通过从小鼠获得的T细胞中免疫相关因子（如TNF-α和IFN-γ）的表达，评估了由[具体名称1]（CV）和[具体名称2]（CP）制备的溶剂提取物的免疫刺激作用。两种[小球藻名称]在己烷和丁醇提取物中均表现出免疫刺激作用，但CV仅增加TNF-α，而CP则同时提高TNF-α和IFN-γ。此外，使用基于非选择性LC-MS/MS的代谢组学分析对[小球藻名称]的脂质谱进行了表征。根据脂质组学结果，这两个物种在脂质组成上存在差异，PC 32:1、PC 32:2、PC 34:5、DGDG 34:4和DGDG 34:5在CV的活性提取物中富集，而PC 34:1、PC 34:2、PC O-15:2和DGTS 34:4在CP的提取物中含量丰富。此外，在两种[小球藻名称]中均检测到的5'-甲硫腺苷（MTA）也表现出免疫刺激特性。
