Chen Zibei, Wang Chenguang, Wang Kai, Sun Yujian, Zhang Yihan, Yuan Xijun, Wang Lina, Bai Qiang, Chen Dehong, Sui Ning
College of Environment and Safety Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China; College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China.
College of Materials Science and Engineering, Qingdao University of Science and Technology, Qingdao, Shandong 266042, China.
J Hazard Mater. 2025 Jun 15;495:138950. doi: 10.1016/j.jhazmat.2025.138950.
The development of high-performance nanozymes is often hindered by intrinsic activity limitations that restrict biosensing sensitivity. In this study, a black phosphorus/graphdiyne (BP/GDY) heterostructure nanozyme was engineered to overcome these challenges, exhibiting significantly enhanced peroxidase (POD)-like activity under light irradiation. Compared to pristine GDY and BP, the BP/GDY nanozyme demonstrated 11.8-fold and 7.7-fold higher catalytic efficiency, respectively. Mechanistic analysis revealed that this enhancement stems from a light-induced structural transformation in GDY (CC → CC transition), which facilitates electron release and optimizes interfacial charge transfer, thus further amplifying POD-like activity. Leveraging this photo-enhanced catalysis and combining the advantages of enzyme-linked immunosorbent assay (ELISA), a high-specificity nanoprobe (BP/GDY-Ab2) was constructed and integrated with a capture antibody to develop a dual-mode biosensing platform for the detection of Vibrio vulnificus (V. Vulnificus). This platform incorporates both colorimetric (LOD = 4.7 CFU mL, linear range: 10-10 CFU mL) and photothermal (LOD = 9.4 CFU mL, linear range: 10-10 CFU mL) signals. The sensor demonstrated high accuracy in detecting V. vulnificus in spiked seafood samples, achieving a rapid response time of 5 min and effectively distinguishing the target pathogen from six interfering bacterial species through spatial charge distribution matching. This study introduces a novel photo-enhanced nanozyme activation strategy, establishing a versatile and scalable platform for on-site food safety monitoring and precision diagnostics, particularly in resource-limited settings.
高性能纳米酶的发展常常受到内在活性限制的阻碍，这些限制会限制生物传感的灵敏度。在本研究中，设计了一种黑磷/石墨炔（BP/GDY）异质结构纳米酶来克服这些挑战，该纳米酶在光照下表现出显著增强的类过氧化物酶（POD）活性。与原始的GDY和BP相比，BP/GDY纳米酶的催化效率分别提高了11.8倍和7.7倍。机理分析表明，这种增强源于GDY中的光诱导结构转变（CC→CC跃迁），这促进了电子释放并优化了界面电荷转移，从而进一步放大了类POD活性。利用这种光增强催化并结合酶联免疫吸附测定（ELISA）的优势，构建了一种高特异性纳米探针（BP/GDY-Ab2），并与捕获抗体整合，开发了一种用于检测创伤弧菌（V. Vulnificus）的双模式生物传感平台。该平台结合了比色（检测限=4.7 CFU mL，线性范围：10-10 CFU mL）和光热（检测限=9.4 CFU mL，线性范围：10-10 CFU mL）信号。该传感器在检测加标海鲜样品中的创伤弧菌时显示出高准确性，实现了5分钟的快速响应时间，并通过空间电荷分布匹配有效地将目标病原体与六种干扰细菌物种区分开来。本研究引入了一种新型的光增强纳米酶激活策略，建立了一个通用且可扩展的平台，用于现场食品安全监测和精准诊断，特别是在资源有限的环境中。
