Pinegar J A, Buxton J D
J Hyg (Lond). 1977 Jun;78(3):387-94. doi: 10.1017/s002217240005628x.
One hundred and thiry-three vanilla slices, purchased from shops in the West Yorkshire Metropolitan County, were examined to determine the numbers and types of bacteria present at the time of purchase. The surface colony count at 37 degrees C was greater than 10(3)/g in 67/133 (50%) of the samples examined, Bacillus cereus being found at that concentration in 21.8%, coliform bacilli including E. coli in 5.3%, Staphylococcus aureus in 3-0% and Streptococcus faecalis in 0-8%. Thirty-four strains of B. cereus were serotyped and 11 (32%) of these were typable with the sera available. Preparation of custard mixed in the laboratory suggests that the milk or milk powder used in the mix may be the major source of B. cereus in the final product. Many of the present methods of manufacture, distribution and storage allow organisms present in the custard at manufacture the opportunity to multiply and possibly reach numbers which present a risk of food poisoning.
从西约克郡都会区的商店购买了133份香草切片,对其进行检查以确定购买时存在的细菌数量和种类。在37摄氏度下,67/133(50%)的检测样本表面菌落数大于10³/g,蜡样芽孢杆菌在该浓度下的检出率为21.8%,包括大肠杆菌在内的大肠菌群为5.3%,金黄色葡萄球菌为3.0%,粪肠球菌为0.8%。对34株蜡样芽孢杆菌进行了血清分型,其中11株(32%)可用现有血清分型。在实验室中混合制作蛋奶沙司表明,混合物中使用的牛奶或奶粉可能是最终产品中蜡样芽孢杆菌的主要来源。目前许多生产、分销和储存方法使制作蛋奶沙司时存在的微生物有机会繁殖,数量可能达到有食物中毒风险的程度。