El-Sayed Ahmed Adel, Elshopakey Gehad, Ateya Ahmed
Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo, 11753, Egypt.
Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Sci Rep. 2025 Jun 20;15(1):20127. doi: 10.1038/s41598-025-04515-1.
The aim of this study was monitoring health status of mastitic Barki ewes using candidate gene approach, gene expression and serum profile of inflammatory and antioxidant markers. A total of 70 ewes were allocated into two equal-sized groups: healthy and ewes have a history of mastitis. DNA sequencing of IFN-γ (365-bp), IL-4 (285-bp), TNF-α (273-bp), MYD88 (660-bp), CCL5 (360-bp), TLR4 (256-bp), TLR9 (414-bp), LTF (299-bp), PRLR (891-bp), CAT (300-bp), GPX1 (221-bp), Keap1 (360-bp), OXSR1 (357-bp), ATOX1 (433-bp), GST (480-bp) and Nrf2 (340-bp) revealed single nucleotide polymorphisms (SNPs) between healthy and mastitic ewes. Levels of IFN-γ, IL-4, TNF-α, MYD88, CCL5, TLR4, TLR9, LTF, PRLR, Keap1 and OXSR1 genes expression were significantly up-regulated in ewes affected with mastitis than resistant ones. Meanwhile CAT, GPX1, ATOX1, GST, and Nrf2 genes elicited an opposite trend. There is a significant elevation of activity of AST, LDH, Hp, CP, SAA, IgG, MDA, and NO levels (P < 0.05), along with reduction of total protein, albumin, GSH, GPx, catalase and SOD (P < 0.05) in mastitic ewes. The findings of this study supported the hypothesis that SNPs in immune and antioxidant genes could be important genetic markers for mastitis susceptibility or resistance in Barki ewes. The examined genes' gene expression profiles may also be utilized as surrogate biomarkers to establish an efficient management regimen and forecast the period of time at which a disease is most likely to manifest.
本研究的目的是采用候选基因方法、基因表达以及炎症和抗氧化标志物的血清谱来监测患乳腺炎的巴尔基母羊的健康状况。总共70只母羊被分为两个大小相等的组：健康母羊和有乳腺炎病史的母羊。对IFN-γ（365 bp）、IL-4（285 bp）、TNF-α（273 bp）、MYD88（660 bp）、CCL5（360 bp）、TLR4（256 bp）、TLR9（414 bp）、LTF（299 bp）、PRLR（891 bp）、CAT（300 bp）、GPX1（221 bp）、Keap1（360 bp）、OXSR1（357 bp）、ATOX1（433 bp）、GST（480 bp）和Nrf2（340 bp）进行DNA测序，结果显示健康母羊和患乳腺炎的母羊之间存在单核苷酸多态性（SNP）。与抗病母羊相比，患乳腺炎的母羊中IFN-γ、IL-4、TNF-α、MYD88、CCL5、TLR4、TLR9、LTF、PRLR、Keap1和OXSR1基因的表达水平显著上调。同时，CAT、GPX1、ATOX1、GST和Nrf2基因呈现相反的趋势。患乳腺炎的母羊中AST、LDH、Hp、CP、SAA、IgG MDA和NO水平的活性显著升高（P<0.05），同时总蛋白、白蛋白、GSH、GPx、过氧化氢酶和SOD降低（P<0.05）。本研究结果支持以下假设：免疫和抗氧化基因中的SNP可能是巴尔基母羊乳腺炎易感性或抗性的重要遗传标记。所检测基因的基因表达谱也可作为替代生物标志物，以建立有效的管理方案并预测疾病最可能出现的时间段。
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