Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo, Egypt.
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Anim Biotechnol. 2023 Apr;34(2):327-339. doi: 10.1080/10495398.2021.1964984. Epub 2021 Aug 18.
The objective of this study was to explore the immunological and antioxidant alterations associated with ovine postpartum disorders. Blood samples were collected from 90 adult Barki ewes and allocated into three equal-sized groups (30 ewes each): control group (CG), inflammatory postpartum disorders group (IPG) and non-inflammatory postpartum disorders group (NIPG). PCR-DNA sequencing approach was carried out for (256-bp) and (456-bp) genes, and nucleotide sequence variations were noticed to be associated with postpartum disorders resistance/susceptibility. Gene expression profile was also evaluated and levels of , , , , and were significantly up-regulated in ewes affected with postpartum disorders than resistant ones, while and genes pattern elicited an opposite trend. Exploring serum profile also showed a significant increase of IL-1α, IL-1β, IL-6, TNF-α, MDA and NO in IPG compared to their correspond values in NIPG and CG. However, serum levels of IL-10, CAT, GSH and GPx were significantly decreased. This study highlights that SNPs in and genes could be genetic markers for postpartum disorders resistance/susceptibility in Barki ewes. Gene expression alongside serum profiles of antioxidant markers could also be used to follow-up the immune status of ewes to build up an effective management protocol.
本研究旨在探讨与绵羊产后疾病相关的免疫和抗氧化改变。从 90 只成年巴吉绵羊中采集血液样本，并将其分为三组（每组 30 只）：对照组（CG）、炎症性产后疾病组（IPG）和非炎症性产后疾病组（NIPG）。通过 PCR-DNA 测序方法对 （256-bp）和 （456-bp）基因进行了研究，发现核苷酸序列变异与产后疾病的抗性/易感性有关。还评估了基因表达谱，发现患有产后疾病的母羊中 、 、 、 、 和 的表达水平显著上调，而 和 基因的表达模式则呈现相反的趋势。对血清谱的探索也表明，与 NIPG 和 CG 相比，IPG 中的 IL-1α、IL-1β、IL-6、TNF-α、MDA 和 NO 显著增加。然而，IL-10、CAT、GSH 和 GPx 的血清水平显著降低。本研究表明， 和 基因中的 SNP 可能是巴吉绵羊产后疾病抗性/易感性的遗传标记。抗氧化标记物的基因表达和血清谱也可用于监测绵羊的免疫状态，以建立有效的管理方案。
