Hiranmayee Gottumukkala, Mallick Sarada Prasanna, Reddy Golamari Siva
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, 522 502, India.
Department of Biotechnology, National Institute of Technology, Tadepalligudem, Andhra Pradesh, 534101, India.
Bioresour Bioprocess. 2025 Jun 21;12(1):67. doi: 10.1186/s40643-025-00853-0.
Plant probiotics are bacteria that play a significant role in enhancing plant growth and health. To understand the interactions between plant probiotics and host plants, a comprehensive approach of antagonistic activity and analytical methods such as high-performance liquid chromatography (HPLC), gas chromatography‒mass spectrometry (GC‒MS), and Fourier transform infrared (FT‒IR) spectroscopy, were employed. The previously isolated bacterial strains, namely, Corynebacterium accolens strain CNTC Th1/57, Bacillus rugosus strain SPB7, Lactobacillus pasteurii DSM 23907 and Cytobacillus firmus strain NBRC 15306, were exposed to antagonistic testing against Botrytis cinerea and Fusarium oxysporum. Considering the results of the antagonistic activity both in vitro and statistically, the bacterial strains Bacillus rugosus strain SPB7 and Lactobacillus pasteurii DSM 23907 presented greater zones of inhibition. Hence these bacteria were moved to obtain comprehensive insights into the chemical composition. HPLC and GC‒MS resulted in the identification of phenols and organic acids. These results were further confirmed by FT-IR, which revealed a peak at 3500 cm for Bacillus rugosus strain SPB7, where O-H, aromatic C-H and aromatic C = C stretching vibrations were also observed at 3069 and 1549 cm. The peak at 1736 cm corresponds to the carboxyl group (-COOH) as the functional group with respect to Lactobacillus pasteurii DSM 23907. Further confirmation was performed by observing the other absorption bands at 3451 cm and 2958 cm, indicating the presence of hydroxyl group (O-H) and alkyl group (C-H) stretching vibrations, thus confirming their potential for the production of phenols and organic acids, respectively, by bacteria. This findings would make a way to explore plant diseases, tolerance against pathogens, and also study ecological role of these bacteria in plant communities.
植物益生菌是在促进植物生长和健康方面发挥重要作用的细菌。为了了解植物益生菌与宿主植物之间的相互作用，采用了拮抗活性的综合方法以及高效液相色谱（HPLC）、气相色谱 - 质谱联用（GC - MS）和傅里叶变换红外（FT - IR）光谱等分析方法。先前分离出的细菌菌株，即簇拥棒状杆菌菌株CNTC Th1/57、皱纹芽孢杆菌菌株SPB7、巴斯德乳杆菌DSM 23907和坚强芽孢杆菌菌株NBRC 15306，针对灰葡萄孢菌和尖孢镰刀菌进行了拮抗测试。综合考虑体外拮抗活性结果和统计学结果，皱纹芽孢杆菌菌株SPB7和巴斯德乳杆菌DSM 23907表现出更大的抑菌圈。因此，对这些细菌进行进一步研究以全面了解其化学成分。HPLC和GC - MS鉴定出了酚类和有机酸。FT - IR进一步证实了这些结果，其显示皱纹芽孢杆菌菌株SPB7在3500 cm处有一个峰，在3069和1549 cm处还观察到了O - H、芳香族C - H和芳香族C = C伸缩振动。对于巴斯德乳杆菌DSM 23907，1736 cm处的峰对应于羧基（-COOH）官能团。通过观察3451 cm和2958 cm处的其他吸收带进行了进一步确认，表明存在羟基（O - H）和烷基（C - H）伸缩振动，从而分别证实了这些细菌产生酚类和有机酸的潜力。这些发现将为探索植物病害、对病原体的耐受性以及研究这些细菌在植物群落中的生态作用开辟道路。
