Sanchez A
FEBS Lett. 1985 Oct 28;191(2):283-6. doi: 10.1016/0014-5793(85)80025-9.
The secretion evoked in human platelets by physiological agonists is an energy-requiring process that depends on the metabolic ATP pool. Diacylglycerol, phorbol ester and collagen evoke a secretory response, which is not associated with an increase of cell Ca2+ levels, and is attributed to activation of protein kinase C [(1983) Nature 305, 317-319]. The secretion evoked by these agonists decreased along with cytoplasmic ATP depletion in the same way that the thrombin-induced secretion did. The secretory response was restored by raising again the cytoplasmic ATP levels. These results support the idea that the secretory response takes place by the physiological ATP-dependent mechanisms rather than by membrane perturbations in these instances.
生理激动剂在人血小板中引发的分泌是一个需要能量的过程，该过程依赖于代谢ATP池。二酰基甘油、佛波酯和胶原蛋白引发分泌反应，此反应与细胞Ca2+水平的升高无关，并且归因于蛋白激酶C的激活[（1983年）《自然》305卷，317 - 319页]。这些激动剂引发的分泌与凝血酶诱导的分泌一样，随着细胞质ATP的消耗而减少。通过再次提高细胞质ATP水平，分泌反应得以恢复。这些结果支持了这样一种观点，即在这些情况下，分泌反应是通过生理上依赖ATP的机制发生的，而不是通过膜扰动发生的。
